Membrane Protein Structure and Function Characterization

Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2.- Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex.- Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris protoplasts.- Cell-free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-synthase subunits.- Wheat Germ Cell Free Overexpression for the Production of Membrane Proteins.- Methyl-specific Isotope Labelling Strategies for NMR Studies of Membrane Proteins.- Labeling of Membrane Complexes for Electron Microscopy.- Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins Case Study of The Mouse 5-HT Receptor.- Characterization of New Detergent and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization.- Secondary Structure Determination by Means of ATR-FTIR Spectroscopy.- Native Mass Spectroscopy forthe Characterization of Structure and Interactions of Membrane Proteins.- Mass Spectroscopy of Mitochondrial Membrane Proteins Complexes.- Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+NQR from V cholerae in the Presence of Lipids.- Reconstitution of Membrane Protein in Liposomes.- Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes.- The CRACAM Robot: Two Dimensional Crystallization of Membrane Protein.- Reconstitution of Membrane Proteins into Nanodiscs for Single Particle Electron Microscopy.- Solid-state NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO.- Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.- Simulation of Ligand Binding To Membrane Proteins.- Molecular modelling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO