Malaria Methods and Protocols
(Sprache: Englisch)
The Plasmodium spp. parasite was identified as the causative agent of malaria in 1880, and the mosquito was identified as the vector in 1897. Despite subsequent efforts focused on the epidemiology, cell biology, immunology, molecular biology, and clinical...
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The Plasmodium spp. parasite was identified as the causative agent of malaria in 1880, and the mosquito was identified as the vector in 1897. Despite subsequent efforts focused on the epidemiology, cell biology, immunology, molecular biology, and clinical manifestations of malaria and the Plasmodium parasite, there is still no licensed vaccine for the prevention of malaria. Physical barriers (bed nets, window screens) and chemical prevention methods (insecticides and mosquito repellents) intended to interfere with the transmission of the disease are not highly effective, and the profile of resistance of the parasite to chemoprophylactic and chemotherapeutic agents is increasing. The dawn of the new millennium has seen a resurgence of interest in the disease by government and philanthropic organizations, but we are still faced with compl- ities of the parasite, the host, and the vector, and the interactions among them. Malaria Methods and Protocols offers a comprehensive collection of protocols describing conventional and state-of-the-art techniques for the study of malaria, as well as associated theory and potential problems, written by experts in the field. The major themes reflected here include assessing the risk of infection and severity of disease, laboratory models, diagnosis and typing, molecular biology techniques, immunological techniques, cell biology techniques, and field applications.
Despite considerable scientific and medical effort over the past decades, malaria remains the most important human parasitic disease. It is responsible for up to 3 million deaths and another 300-500 million new cases each year, and is becoming resistant to the current chemoprophylactic and chemotherapeutic agents. In Malaria Methods and Protocols, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. Areas covered include clinical and laboratory diagnosis and typing, animal models, molecular biology, immunology, cell biology, vaccinology, laboratory models, and field applications. Each readily reproducible protocol has been tested, standardized, and optimized for experimental success, and includes many laboratory notes on troubleshooting, avoiding pitfalls, and interpreting results. Several of the most widely used methods are either described here in detail for the first time or have been thoroughly updated since their original publication (e.g., in vitro culture of Plasmodium parasites and in vitro growth inhibition assay).
State-of-the-art and highly practical, Malaria Methods and Protocols makes available to basic and applied researchers today's only comprehensive collection of essential laboratory methods for diagnosing malaria, characterizing the parasite, understanding the interaction between the human host and Plasmodium parasite, and developing effective preventive measures.aria. Areas covered include clinical and laboratory diagnosis and typing, animal models, molecular biology, immunology, cell biology, vaccinology, laboratory models, and field applications. Each readily reproducible protocol has been tested, standardized, and optimized for experimental success, and includes many laboratory notes on troubleshooting, avoiding pitfalls, and interpreting results. Several of the most widely used methods are either described here in detail for the first time or have been thoroug
State-of-the-art and highly practical, Malaria Methods and Protocols makes available to basic and applied researchers today's only comprehensive collection of essential laboratory methods for diagnosing malaria, characterizing the parasite, understanding the interaction between the human host and Plasmodium parasite, and developing effective preventive measures.aria. Areas covered include clinical and laboratory diagnosis and typing, animal models, molecular biology, immunology, cell biology, vaccinology, laboratory models, and field applications. Each readily reproducible protocol has been tested, standardized, and optimized for experimental success, and includes many laboratory notes on troubleshooting, avoiding pitfalls, and interpreting results. Several of the most widely used methods are either described here in detail for the first time or have been thoroug
Inhaltsverzeichnis zu „Malaria Methods and Protocols “
Assessing Risk of Infection and Severity of Disease.- Vector Incrimination and Entomological Inoculation Rates.- Epidemiological Measures of Risk of Malaria.- Laboratory Models.- Maintenance of the Plasmodium berghei Life Cycle.- Mouse Models for Pre-Erythrocytic-Stage Malaria.- Mouse Models for Erythrocytic-Stage Malaria.- Nonhuman Primate Models.- Nonhuman Primate Models.- Diagnosis and Typing.- Vector Analysis.- Genotyping of Plasmodium spp..- Genotyping of Plasmodium falciparum.- Microsatellite Analysis in Plasmodium falciparum.- Quantitation of Liver-Stage Parasites by Automated TaqMan® Real-Time PCR.- Quantitation of Liver-Stage Parasites by Competitive RT-PCR.- Molecular Biology Techniques.- Extraction and Purification of Plasmodium Total RNA.- Extraction and Purification of Plasmodium Parasite DNA.- Southern Blotting of Parasite DNA.- SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins.- Nested PCR Analysis of Plasmodium Parasites.- RFLP Analysis.- Analysis of Gene Expression by RT-PCR.- In Situ Detection of RNA in Blood- and Mosquito-Stage Malaria Parasites.- Purification of Chromosomes from Plasmodium falciparum.- Construction of Genomic Libraries from the DNA of Plasmodium Species.- Construction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNA.- Construction of Plasmodium falciparum ? cDNA Libraries.- Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization.- Construction and Screening of YAC Libraries.- Episomal Transformation of Plasmodium berghei.- Gene Targeting in Plasmodium berghei.- Immunological Techniques.- Peptide Vaccination.- DNA Vaccination.- Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses.- Assessing CD4+HelperT-Lymphocyte Responses by Lymphoproliferation.- Limiting Dilution Analysis of Antigen-Specific CD4+ T-Cell Responses in Mice.- Cell Trafficking.- Assessing Antigen-Specific Proliferation and Cytokine Responses
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Using Flow Cytometry.- Cytokine Analysis by Intracellular Staining.- Assessment of Antigen-Specific CTL- and CD8+-Dependent IFN-? Responses in Mice.- Assessing Antigen-Specific CD8+ CTL Responses in Humans.- Human Antibody Subclass ELISA.- Systemic Nitric Oxide Production in Human Malaria.- Systemic Nitric Oxide Production in Human Malaria.- Cell Biology Techniques.- In Vitro Culture of Plasmodium Parasites.- Automated Synchronization of Plasmodium falciparum Parasites by Culture in a Temperature-Cycling Incubator.- Hepatic Portal Branch Inoculation.- Hepatocyte Perfusion, Isolation, and Culture.- Inhibition of Sporozoite Invasion.- Inhibition of Liver-Stage Development Assay.- T-Cell Mediated Inhibition of Liver-Stage Development Assay.- Antibody-Dependent Cellular Inhibition Assay.- Erythrocytic Malaria Growth or Invasion Inhibition Assays with Emphasis on Suspension Culture GIA.- Analysis of CSA-Binding Parasites and Antiadhesion Antibodies.- Analysis of the Adhesive Properties of Plasmodium falciparum-Infected Red Blood Cells Under Conditions of Flow.- Preparation of Adhesive Targets for Flow-Based Cytoadhesion Assays.- Triton X-114 Phase Partitioning for Antigen Characterization.- Immunoprecipitation for Antigen Localization.- Testing Interventions For Malaria Control.- Field Trials.
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Bibliographische Angaben
- 2010, XV, 631 Seiten, Masse: 17,9 x 25,4 cm, Kartoniert (TB), Englisch
- Herausgegeben: Denise L. Doolan
- Verlag: Springer, Berlin
- ISBN-10: 1617372056
- ISBN-13: 9781617372056
Sprache:
Englisch
Pressezitat
"In this book, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria." - Clinical Laboratory International"This is an outstanding collection of protocols written by over eighty experts in the field. The geographical spread of these contributors is impressive, encompassing writers from thirteen countries on six continents. There is good stylistic continuity throughout the book, despite the diversity of the authors...there is admirable cross-referencing between chapters." -Molecular Biotechnology
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