Lipidomics
Volume 2: Methods and Protocols
(Sprache: Englisch)
Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of...
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Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures. Volume 2 focuses on oxidized lipids, trafficking and profiling, software, bioinformatics, and biostatistics. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.
Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.
Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.
Klappentext zu „Lipidomics “
Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures. Volume 2 focuses on oxidized lipids, trafficking and profiling, software, bioinformatics, and biostatistics. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.
Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures. Volume 2 focuses on oxidized lipids, trafficking and profiling, software, bioinformatics, and biostatistics. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.
Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.
Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.
Inhaltsverzeichnis zu „Lipidomics “
Part I: Oxidized Lipids1. F2-Isoprostanes: Sensitive Biomarkers of Oxidative Stress in Vitro and in Vivo: A Gas Chromatography-Mass Spectrometric Approach Ingrid Wiswedel
2. Volatile Oxylipins and Related Compounds Formed Under Stress in Plants Kenji Matsui, Koichi Sugimoto, Pattana Kakumyan, Sergey A. Khorobrykh, and Jun'ichi Mano
3. Quantification of Lysophosphatidylcholine Species by High Throughput Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS) Gerhard Liebisch and Gerd Schmitz
4. Use of Lipidomics for Analyzing Glycerolipid and Cholesteryl Ester Oxidation by Gas Chromatography, HPLC, and On-line MS Arnis Kuksis, Jukka-Pekka Suomela, Marko Tarvainen, and Heikki Kallio
5. Lipid Raft Redox Signaling Platforms in Plasma Membrane Fan Yi, Si Jin, and Pin-Lan Li
6. Mass Spectrometry Analysis of Polyisoprenoids Alcohols and Carotenoids via ESI(Li+)-MS/MS Fabio Luiz D'Alexandri, Renata Tonhosolo, Emilia A. Kimura, and Alejandro Miguel Katzin
7. Detection of a Lipid-Lysine Adduct Family with an Amide-Bond as the Linkage:
- Novel Markers for Lipid-Derived Protein Modifications Yoji Kato and Toshihiko Osawa
8. Assessing the Neuroprotective Effect of Antioxidative Food Factors by Application of Lipid-Derived Dopamine Modification Adducts Xuebo Liu, Naruomi Yamada, and Toshihiko Osawa
9. Mass-Spectrometric Characterization of Phospholipids and Their Hydroperoxide-Derivatives in Vivo: Effects of Total Body Irradiation Yulia Y. Tyurina, Vladimir A. Tyurin, Valentina I. Kapralova, Andrew A. Amoscato, Michael W. Epperly, Joel S. Greenberger, and Valerian E. Kagan
Part II: Trafficking and Profiling
10. Comprehensive mRNA Profiling of Lipid-Related Genes in Microglia and Macrophages Using Taqman Arrays Richard Mauerer, Yana Walczak, and Thomas Langmann
11. Imaging Lipid Membrane Domains with Lipid-Specific Probes Françoise Hullin-Matsuda, Reiko Ishitsuka, Miwa Takahashi and Toshihide Kobayashi
12. Monitoring Sterol Uptake,
... mehr
Acetylation, and Export in Yeast Vineet Choudhary and Roger Schneiter
13. Methods to Monitor Fatty Acid Transport Proceeding Through Vectorial Acylation Elsa Arias-Barrau, Concetta C. DiRusso, and Paul N. Black
14. Activity-Based Profiling of Lipases in Living Cells Maximilian Schicher, Iris Jesse, and Ruth Birner-Gruenberger
15. Histochemistry and Lipid Profiling Combine for Insights into Aging and Age-related Maculopathy Christine A. Curcio, Martin Rudolf, and Lan Wang
Part III: Software and Bioinformatics
16. Instrument-Independent Software Tools for the Analysis of MS/MS and LC-MS Lipidomics Data Perttu Haimi, Krishna Chaithanya B., Ville Kainu, Martin Hermansson, and Pentti Somerharju
17. Computer-Assisted Interpretation of Triacylglycerols Mass Spectra Josef Cvacka and Edita Kofronová
18. Visualization of Complex Processes in Lipid Systems Using Computer Simulations and Molecular Graphics Jelena Telenius, Ilpo Vattulainen, and Luca Monticelli
19. Bioinformatics Strategies for the Analysis of Lipids Craig E. Wheelock, Susumu Goto, Laxman Yetukuri, Fabio Luiz D'Alexandri, Christian Klukas, Falk Schreiber, and Matej Oresic
Part IV: Biostatistics
20. The Effect of Lipid Adjustment on the Analysis of Environmental Contaminants and the Outcome of Human Health Risks Audrey J. Gaskins and Enrique F. Schisterman Christine A. Curcio, Martin Rudolf, and Lan Wang
Part III: Software and Bioinformatics
16. Instrument-Independent Software Tools for the Analysis of MS/MS and LC-MS Lipidomics Data Perttu Haimi, Krishna Chaithanya B., Ville Kainu, Martin Hermansson, and Pentti Somerharju
17. Computer-Assisted Interpretation of Triacylglycerols Mass Spectra Jos
13. Methods to Monitor Fatty Acid Transport Proceeding Through Vectorial Acylation Elsa Arias-Barrau, Concetta C. DiRusso, and Paul N. Black
14. Activity-Based Profiling of Lipases in Living Cells Maximilian Schicher, Iris Jesse, and Ruth Birner-Gruenberger
15. Histochemistry and Lipid Profiling Combine for Insights into Aging and Age-related Maculopathy Christine A. Curcio, Martin Rudolf, and Lan Wang
Part III: Software and Bioinformatics
16. Instrument-Independent Software Tools for the Analysis of MS/MS and LC-MS Lipidomics Data Perttu Haimi, Krishna Chaithanya B., Ville Kainu, Martin Hermansson, and Pentti Somerharju
17. Computer-Assisted Interpretation of Triacylglycerols Mass Spectra Josef Cvacka and Edita Kofronová
18. Visualization of Complex Processes in Lipid Systems Using Computer Simulations and Molecular Graphics Jelena Telenius, Ilpo Vattulainen, and Luca Monticelli
19. Bioinformatics Strategies for the Analysis of Lipids Craig E. Wheelock, Susumu Goto, Laxman Yetukuri, Fabio Luiz D'Alexandri, Christian Klukas, Falk Schreiber, and Matej Oresic
Part IV: Biostatistics
20. The Effect of Lipid Adjustment on the Analysis of Environmental Contaminants and the Outcome of Human Health Risks Audrey J. Gaskins and Enrique F. Schisterman Christine A. Curcio, Martin Rudolf, and Lan Wang
Part III: Software and Bioinformatics
16. Instrument-Independent Software Tools for the Analysis of MS/MS and LC-MS Lipidomics Data Perttu Haimi, Krishna Chaithanya B., Ville Kainu, Martin Hermansson, and Pentti Somerharju
17. Computer-Assisted Interpretation of Triacylglycerols Mass Spectra Jos
... weniger
Bibliographische Angaben
- 2009, Repr. d. Ausg. v. 2009, XII, 390 Seiten, 5 farbige Abbildungen, Masse: 19,8 x 26,6 cm, Gebunden, Englisch
- Herausgegeben: Donald Armstrong
- Verlag: Springer, Berlin
- ISBN-10: 1607613247
- ISBN-13: 9781607613244
- Erscheinungsdatum: 29.10.2009
Sprache:
Englisch
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