Laboratory Protocols in Fungal Biology

Preface.- Long Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results.- Confocal Laser and Epifluorescence Microscopy.- Diagnose of Chytid Parasites of Algae in The Plankton.- Sabouraud Agar for Fungal Growth.- FISH, Fluorescence in Situ Hybridization of Uncultured Zoosporic Fungi.- Real-time Quantitative PCR Assay for Counting Uncultured Zoosporic Fungi.- Real-Time PCR Assay in Fungi.- Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes.- Extraction and Characterization of Taxol: An Anticancer Drug from an Endophytic And Pathogenic Fungi.- Recent Advances in Applications of Machine Learning in Fungal Biology.- Fungal Conservation.- TBD.- Loop-Mediated Isothermal Amplification of the Entomopathogenic Fungi, Beauveria bassiana.- Molecular Taxonomy and Multi-Gene Phylogeny of Fungi.- Yeast Isolation Methods from Specialized Habitats.- Assays for the Quantification of Antioxidant Enzymes in Fungi.- Index.

Vijai Kumar Gupta is currently working as Senior Fellow at Center for Safe and Improved Foods & Biorefining and Advanced Materials Research Center, Scotland's Rural College (SRUC), Edinburgh, UK. Dr Gupta received his doctoral degree in Microbiology in 2009 from Dr RML Avadh University India, and the leading expert in the area of Biomass Valorisation, Microbial Bioprocess Biotechnologies, Bioactive Natural Products, and Environmental Sustainability.

He is the Secretary of the European Mycological Association and also the International Professor at IFST-China, Visiting Professor at SCBG-China; SZIU-Hungary; SHUATS-India. Dr Gupta is the Fellow of few international scientific societies including- Linnaean Society-UK; Mycological Society of India; Indian Mycological Association; Academy of Microbiological Sciences India; National Academy of Agricultural Sciences, India and Member of Royal Society Biology, UK. He has 175 peer-reviewed publications, 33 book chapters and 39 books in his hands with author h-index 50, i10-index 173 with total 11K+ citations to-date. Also, he is the editor of few reputed journals including, Microbial Cell Factories, Scientific Reports, Microbiome, Biotechnology Advances and Critical Reviews in Biotechnology.

Maria Tuohy is the Principal Investigator and Head of the Molecular Glycobiotechnology Group, School of Natural Sciences, NUI, Galway, Ireland. Dr. Tuohy has a strong track record in Glycobiotechnology and Enzyme Biotechnology, with ~28 years' experience in fungal biology and biotechnology. A special focus of the research is on developing enabling technology for bioconversion of terrestrial plants and algae for biorefinery and bioenergy applications. This research encompasses protein biotechnology, molecular enzymology, proteomics, fungal genetics, biology and genomics, fermentation, analytical methods in glycobiology and protein biochemistry, development of novel energy systems, as well as key