Kidney Research

Isolation and Propagation of Cell Populations.- Isolation and Propagation of Glomerular Mesangial Cells.- Isolation and Primary Culture of Human Proximal Tubule Cells.- Propagation and Culture of Renal Fibroblasts.- Animal Models.- Small Animal Models of Kidney Disease: A Review.- Techniques and Applications.- Measurement of Glomerular Filtration Rate in Conscious Mice.- Laser Capture Microdissection of Kidney Tissue.- Quantitative Gene Expression Analysis in Kidney Tissues.- In Vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy.- Using In Situ Hybridisation to Localize Renal Gene Expression in Tissue Sections.- Immuno and Lectin Histochemistry for Renal Light Microscopy.- Immuno and Lectin Histochemistry for Renal Electron Microscopy.- Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia.- Identification of Apoptosis in Kidney Tissue Sections.- Cell-Populated Floating Collagen Lattices: An In Vitro Model of Parenchymal Contraction.- Mechanical Stretch-Induced Signal Transduction in Cultured Mesangial Cells.- Determination of Collagen Content, Concentration, and Sub-types in Kidney Tissue.- SELDI-TOF Mass Spectrometry-Based Protein Profiling of Kidney Tissue.- In Vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli.

From the reviews:"This book details research methodologies especially relevant for nephrological studies. ... Researchers in the field of nephrology are the intended audience. The book covers research methodologies used in renal studies (as well as in other studies), including proteomics, micro-RNA work, and other cutting-edge approaches. ... This is a unique book that has no competitors." (Jens Goebel, Doody's Book Reviews, December, 2011)