In Vitro Mutagenesis Protocols

Mutagenesis in Various Microbial Backgrounds.- Mutagenesis Protocols in Saccharomyces cerevisiae by In Vivo Overlap Extension.- In Vitro Mutagenesis of Brucella Species.- Random Mutagenesis Strategies for Campylobacter and Helicobacter Species.- Mutagenesis of the Repeat Regions of Herpesviruses Cloned as Bacterial Artificial Chromosomes.- An Efficient Protocol for VZV BAC-Based Mutagenesis.- A Method for Rapid Genetic Integration into Plasmodium falciparum Utilizing Mycobacteriophage Bxb1 Integrase.- PCR Mutagenesis.- Random Mutagenesis by Error-Prone PCR.- A Rapid and Versatile PCR-Based Site-Directed Mutagenesis Protocol for Generation of Mutations Along the Entire Length of a Cloned cDNA.- Rapid Sequence Scanning Mutagenesis Using In Silico Oligo Design and the Megaprimer PCR of Whole Plasmid Method (MegaWHOP).- Insertion and Deletion Mutagenesis by Overlap Extension PCR.- Targeted Amplification of Mutant Strands for Efficient Site-Directed Mutagenesis and Mutant Screening.- A Modified Inverse PCR Procedure for Insertion, Deletion, or Replacement of a DNA Fragment in a Target Sequence and Its Application in the Ligand Interaction Scan Method for Generation of Ligand-Regulated Proteins.- Amplification of Orthologous Genes Using Degenerate Primers.- Reviews.- Computational Evaluation of Protein Stability Change upon Mutations.- Approaches for Using Animal Models to Identify Loci That Genetically Interact with Human Disease-Causing Point Mutations.- Protein Evolution Mutagenesis.- Using Peptide Loop Insertion Mutagenesis for the Evolution of Proteins.- Massive Mutagenesis®: High-Throughput Combinatorial Site-Directed Mutagenesis.- Directed In Vitro Evolution of Reporter Genes Based on Semi-Rational Design and High-Throughput Screening.- Ribosome Display for RapidProtein Evolution by Consecutive Rounds of Mutation and Selection.- Protein Structure and Function Mutagenesis.- Fine-Tuning Enzyme Activity Through Saturation Mutagenesis.- Characterization of Structural

From the reviews of the third edition:"This well-illustrated book details specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein-structure function, protein regulation of biological processes, and protein activity and mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. ... Scientists, students, and others interested in the field of in vitro mutagenesis will find this extremely useful. ... This book successfully describes a variety of in vitro mutagenesis protocols for scientists working in this area." (Omer Iqbal, Doody's Review Service, November, 2010)