Histone Deacetylases

A Sensitive and Flexible Assay for Determining Histone Deacetylase 1 (HDAC1) Activity.- Detection of Sumo Modification of Endogenous Histone Deacetylase 2 (HDAC2) in Mammalian Cells.- Analysis of Epigenetic Regulation of Hypoxia-Induced Epithelial-Mesenchymal Transition in Cancer Cells by Quantitative Chromatin Immunoprecipitation of Histone Deacetylase 3 (HDAC3).- Molecular and Functional Characterization of Histone Deacetylase 4 (HDAC4).- Approaches for Studying the Subcellular Localization, Interactions, and Regulation of Histone Deacetylase 5 (HDAC5).- Analysis of Expression and Functions of Histone Deacetylase 6 (HDAC 6).- Analysis of Histone Deacetylase 7 (HDAC7) Alternative Splicing and Its Role in Embryonic Stem Cell Differentiation toward Smooth Muscle Lineage.- Large-Scale Overproduction and Purification of Recombinant Histone Deacetylase 8 (HDAC8) from the Human-Pathogenic Flatworm Schistosoma mansoni.- Visualization of Histone Deacetylase9 (HDAC9) Spatiotemporal Subcellular Localization in Primary Neuron Cultures.- Expression and Function of Histone Deacetylase 10 (HDAC10) in B Cell Malignancies.- Functional Analysis of Histone Deacetylase 11 (HDAC11).- Sirtuin1 (SIRT1) in the Acetylation of Downstream Target Proteins.- Protocols for Cloning, Expression, and Functional Analysis of Sirtuin2 (SIRT2).- Cloning and Characterization of Sirtuin3 (SIRT3).- Identification of Sirtuin4 (SIRT4) Protein Interactions: Uncovering Candidate Acyl-Modified Mitochondrial Substrates and Enzymatic Regulators.- Generation and Purification of Catalytically Active Recombinant Sirtuin5 (SIRT5) Protein.- Sirtuin6 (SIRT6) Activity Assays.- Molecular, Cellular, and Physiological Characterization of Sirtuin7 (SIRT7).- HDAC Inhibitors.- Assessment of the Anti-Proliferative Activity of a BET Bromodomain Inhibitor as Single Agent and in Combination in Non-Hodgkin Lymphoma Cell Lines.- Screening the

"This volume of Methods in Molecular Biology, Histone deacetylases, provides comprehensive overview of the methods and procedures for analysis of histone modifications, namely histone deacetylation. ... Methodological tools required for more detailed determination of the molecular and biological roles of distinct HDACs and HDAC-containing complexes are summarized in this book, which will no doubt hasten these important mechanistic studies." (Bozena Smolkova, Neoplasma, Vol. 64 (3), 2017)