Guide to Techniques in Mouse Development, Part B

Section I. Transgenesis
1. Lentivirus transgenesis
Alexander Pfeifer and Katrin Zimmermann
2. Germline modification using mouse spermatogonial stem cells
Mito Kanatsu-Shinohara and Takashi Shinohara
3. Embryonic in vivo electroporation in the mouse
Tetsuichiro Saito

Section II. Transposons
4. Current applications of transposons in mouse genetics
Adam J. Dupuy
5. Functional genomics in the mouse using the Sleeping Beauty transposon system
Kyoji Horie, Chikara Kokubu, and Junji Takeda
6. The use of DNA transposons for cancer gene discovery in mice
George Vassiliou, Roland Rad, and Allan Bradley

Section III. Recombinases
7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2
Konstantinos Anastassiadis, Stefan Glaser, Andrea Kranz, Kaj Berhardt, and A. Francis Stewart
8. A recombineering pipeline to make conditional targeting constructs
Jun Fu, Madeleine Teucher, Konstantinos Anastassiadis, William Skarnes, and A. Francis Stewart
9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria
M. David Stewart and Richard R. Behringer
10.Genetic fate mapping using site-specific recombinases
Emilie Legué and Alexandra L. Joyner
11. Mapping cell fate and function using recombinase-based intersectional strategies
Susan M. Dymecki, Russell S. Ray, and Jun C. Kim

Section IV. Mutagenesis
12. Genome-wide forward genetic screens in mouse ES cells
Meng Amy Li, Stephen J. Pettitt, Kosuke Yusa, and Allan Bradley
13. Gene trap mutagenesis in the mouse
Roland H. Friedel and Philippe Soriano
14. A wider context for gene trap mutagenesis
Joshua M. Brickman, Anestis Tsakiridis, Christine To, and William L. Stanford
15. Mouse mutagenesis with the chemical supermutagen ENU
Frank J. Probst and Monica J. Justice
16. Phenotype-driven mouse ENU mutagenesis screens
Tamara Caspary
17. Using ENU mutagenesis for