Germline Development

Isolation of Fetal Gonads from Embryos of Timed-Pregnant Mice for Morphological and Molecular Studies.- Neonatal Testicular Gonocytes Isolation and Processing for Immunocytochemical Analysis.- Isolation of Undifferentiated and Early Differentiating Type A Spermatogonia from Pou5f1-GFP Reporter Mice.- Isolation of Human Male Germline Stem Cells Using Enzymatic Digestion and Magnetic-Activated Cell Sorting.- Isolation and Purification of Murine Male Germ Cells.- Preparation of Enriched Mouse Syncitia-Free Pachytene Spermatocyte Cell Suspensions.- Revealing Transcriptome Landscape of Mouse Spermatogonial Cells by Tiling Microarray.- Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha Acetyltransferase.- Identification of Novel Long Non-Coding RNA Transcripts in Male Germ Cells.- Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization.- Spermatogenesis in Cryptorchidism.- In Vitro Culture of Fetal Ovaries: A Model to Study Factors Regulating Early Follicular Development.- Microspread Oocyte Preparations for the Analysis of Meiotic Prophase Progression with Improved Recovery by Cytospin Centrifugation.- In Vitro Maturation (IVM) of Porcine Oocytes.- Experimental Approaches to the Study of Human Primordial Germ Cells.- Investigating the Origins of Somatic Cell Populations in the Perinatal Mouse Ovaries Using Genetic Lineage Tracing and Immunohistochemistry.- DNA Methylation Analysis of Germ Cells by Using Bisulfite-Based Sequencing Methods.- Gene Expression in Mouse Oocytes by RNA-Seq.