Gene Therapy Protocols

1 Preparation and Quantification of Pseudotyped Retroviral Vector Hong Yu and Young Jik Kwon

2 Production of Retroviral Vectors for Clinical Use Kenneth Cornetta, Lilith Reeves and Scott Cross

3 Methods for the Production of Helper-Dependent Adenoviral Vectors Donna J. Palmer and Philip Ng

4 Methods for the Production of First Generation Adenoviral Vectors Donna J. Palmer and Philip Ng

5 Large Scale Production of Recombinant Adeno-Associated Vectors Alejandro Negrete and Robert M. Kotin

6 Construction and Production of Recombinant Herpes Simplex Virus Vectors William F. Goins, David M. Krisky, James B. Wechuck, Shaohua Huang and Joseph C. Glorioso

7 Plasmid-based Gene Transfer in Mouse Skeletal Muscle by Electroporation Johnathan D. Schertzer and Gordon S. Lynch

8 Chitosan Nanoparticle-Mediated Gene Transfer Dongwon Lee and Shyam S. Mohapatra

9 PEG-PEI Copolymers for Oligonucleotide Delivery to Cells and Tissues Gordon J. Lutz, Shashank R. Sirsi and Jason H. Williams

10 Non-Viral Gene Delivery with Cationic Liposome-DNA Complexes Kai K. Ewert, Ayesha Ahmad, Nathan F. Bouxsein, Heather M. Evans and Cyrus R. Safinya

11 Applications of Lentiviral Vectors in Non-invasive Molecular Imaging Abhijit De, Shahriar Shah Yaghoubi and Sanjiv Sam Gambhir

12 Retroviral Modification of Mesenchymal Stem Cells for Gene Therapy of Hemophilia A Christopher B. Doering

13 Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells Lucienne M. Ide, Elisabeth Javazon and H. Trent Spencer

14 Assessment of CFTR Function After Gene Transfer in Vitro and in Vivo Uta Griesenbach,Felix M. Munkonge, Stephanie Sumner-Jones, Emma Holder, Stephen N. Smith, A. Christopher Boyd, Deborah R. Gill, Stephen C. Hyde, David Porteous and Eric WFW Alton

15 Oncolytic Adenoviruses for Cancer Gene Therapy Ta-Chiang Liu, Stephen H. Thorne and David H. Kim

16 Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular

The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. This first volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.