Gene Therapy Protocols

Table of Contents 1 Preparation and Quantification of Pseudotyped Retroviral Vector Hong Yu and Young Jik Kwon 2 Production of Retroviral Vectors for Clinical Use Kenneth Cornetta, Lilith Reeves and Scott Cross 3 Methods for the Production of Helper-Dependent Adenoviral Vectors Donna J. Palmer and Philip Ng 4 Methods for the Production of First Generation Adenoviral Vectors Donna J. Palmer and Philip Ng 5 Large Scale Production of Recombinant Adeno-Associated Vectors Alejandro Negrete and Robert M. Kotin 6 Construction and Production of Recombinant Herpes Simplex Virus Vectors William F. Goins, David M. Krisky, James B. Wechuck, Shaohua Huang and Joseph C. Glorioso 7 Plasmid-based Gene Transfer in Mouse Skeletal Muscle by Electroporation Johnathan D. Schertzer and Gordon S. Lynch 8 Chitosan Nanoparticle-Mediated Gene Transfer Dongwon Lee and Shyam S. Mohapatra 9 PEG-PEI Copolymers for Oligonucleotide Delivery to Cells and Tissues Gordon J. Lutz, Shashank R. Sirsi and Jason H. Williams 10 Non-Viral Gene Delivery with Cationic Liposome-DNA Complexes Kai K. Ewert, Ayesha Ahmad, Nathan F. Bouxsein, Heather M. Evans and Cyrus R. Safinya 11 Applications of Lentiviral Vectors in Non-invasive Molecular Imaging Abhijit De, Shahriar Shah Yaghoubi and Sanjiv Sam Gambhir 12 Retroviral Modification of Mesenchymal Stem Cells for Gene Therapy of Hemophilia A Christopher B. Doering 13 Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells Lucienne M. Ide, Elisabeth Javazon and H. Trent Spencer 14 Assessment of CFTR Function After Gene Transfer in Vitro and in Vivo Uta Griesenbach,Felix M. Munkonge, Stephanie Sumner-Jones, Emma Holder, Stephen N. Smith, A. Christopher Boyd, Deborah R. Gill, Stephen C. Hyde, David Porteous and Eric WFW Alton 15 Oncolytic Adenoviruses for Cancer Gene Therapy Ta-Chiang Liu, Stephen H. Thorne and David H. Kim 16 Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular Dystrophy Gene Therapy Yi