Gene Expression Profiling

Real-Time PCR and Multiplex Approaches.- Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs.- Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals.- Software and Tools for Microarray Data Analysis.- Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumor Cells.- Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay.- MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs.- Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases.- 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins.- Design, Construction, and Analysis of Cell Line Arrays and Tissue Microarrays for Gene Expression Analysis.- Immunohistochemical and Immunofluorescence Procedures for Protein Analysis.- Advanced Microscopy: Laser Scanning Confocal Microscopy.- Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling.- Atomic Force Microscopy (AFM) and High-Content Analysis (HCA): Two Innovative Technologies for Dissecting the Relationship Between Epithelial-Mesenchymal Transition (EMT)-Related Morphological and Structural Alterations and Cell Mechanical Properties.

From the reviews of the second edition:"This beautifully illustrated book on gene expression profiling provides detailed protocols for a broad range of techniques ... for the analysis of gene expression at the DNA, RNA, and protein levels. ... Although written for researchers, the book will be helpful for graduate students and others interested in learning about gene expression. ... This is a valuable resource on methods and protocols necessary to understand the changes in gene expression in normal and pathological states in pursuit of better treatments." (Omer Iqbal, Doody's Book Reviews, April, 2012)