Expression, Purification, and Structural Biology of Membrane Proteins

Cloning and Multi-Subunit Expression of Mitochondrial Membrane Protein Complexes in Saccharomyces cerevisiae.- Membrane Protein Production in Escherichia coli.- Membrane Protein Production in Lactococcus Lactis for Structural Studies.- Expression and Purification of Membrane Proteins in Saccharomyces cerevisiae.- Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System.- Membrane Extracts from Plant Tissues.- Membrane Protein Solubilization and Quality Control: An Example of a Primary Active Transporter.- GPCR Solubilization and Quality Control.- Affinity Purification of Membrane Proteins.- Purification of Membrane Proteins by Affinity Chromatography with On-Column Protease Cleavage.- Biotinylation of Membrane Proteins for Binder Selections.- Production and Application of Nanobodies for Membrane Protein Structural Biology.- Identifying Conformation-Selective Heavy-Chain-Only Antibodies against Membrane Proteins by a Thermal-Shift Scintillation Proximity Assay.- Reconstitution of Membrane Proteins into Platforms Suitable for Biophysical and Structural Analyses.- Reconstitution of Proteoliposomes for Phospholipid Scrambling and Non-Selective Channel Assays.- Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent.- Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs.- Fast Small-Scale Membrane Protein Purification and Grids Preparation for Single Particle Electron Microscopy.- Stabilization and Crystallization of a Membrane Protein Involved in Lipids Transport.- In Meso In Situ Serial X-ray Crystallography (IMISX): A Protocol for Membrane Protein Structure Determination at the Swiss Light Source.- Membrane Protein Preparation for Serial Crystallography Using High Viscosity Injectors: Rhodopsin as an Example.- Hydrogen/Deuterium Exchange Mass Spectrometry for the Structural Analysis of Detergent-Solubilized Membrane Proteins.- Mechanical Unfolding and Refolding of Single Membrane