Enzyme Engineering

A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability in vivo.- Determining Enzyme Kinetics via Isothermal Titration Calorimetry.- GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria.- Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA Binding and Cleavage Properties.- TAL Effector Nuclease (TALEN) Engineering.- In vitro Evolution of Enzymes.- Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In vitro and In vivo.- Reconstructing Evolutionary Adaptive Paths for Protein Engineering.- Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria.- FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants.- Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes.- Gene Synthesis by Assembly of Deoxyuridine Containing Oligonucleotides.- Protein Engineering: Single or Multiple Site-Directed Mutagenesis.- Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination.- Promiscuity-Based Enzyme Selection for Rational Directed Evolution Experiments.- Rational Protein Sequence Diversification by Multi-Codon Scanning Mutagenesis.- Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter.- In Vitro Directed Evolution of Enzymes Expressed by E. coli in Micro-Titre Plates.