Dendritic Cell Protocols

I: Reviews.- Review of Human DC Subtypes.- Review of Murine Dendritic Cells: Types, Location, and Development.- II: Human DC Protocols.- Isolation of Human Blood DC Subtypes.- Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin.- Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells.- In Vivo Modulation of Gene Expression by Lentiviral Transduction in "Human Immune System" Rag2?/??c ?/? Mice.- Generation of Stable Th1/CTL-, Th2-, and Th17-Inducing Human Dendritic Cells.- Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation.- Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines.- III: Murine Dendritic Cell Methods.- CD8+, CD8?, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand.- Generation of Large Numbers of Pro-DCs and Pre-DCs In Vitro.- The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSF.- Isolation of Common Dendritic Cell Progenitors (CDP) from Mouse Bone Marrow.- The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow Cytometry.- Isolation of Cutaneous Dendritic Cells.- Isolation of Skin Dendritic Cells from Mouse and Man.- Identification and Isolation of Rodent Respiratory Tract Dendritic Cells.- Isolation of Mouse Thymic Dendritic Cell Precursors.- Isolation of Rat Intestinal Lymph DC.- Mouse Models of Viral Infection: Influenza Infection in the Lung.- DCs in Mouse Models of Intracellular Bacterial Infection.- Studying the Function of Dendritic Cells in Mouse Models of Asthma.- Direct Ex Vivo Activation of T cells for Analysis of Dendritic Cells Antigen Presentation.-Methods to Study Pulmonary Dendritic Cell Migration.- Measuring pH, ROS Production, Maturation, and Degradation in Dendritic Cell Phagosomes Using Cytofluorometry-Based Assays.- Adaptive Treg Generation by DCs and Their

From the reviews of the second edition:"Written for principal investigators and their students, postdocs, and staff, providing very practical step-by-step instructions on how to work with DC. ... Overall, this book provides a nice compilation of methods for studying dendritic cells. The book will be particularly useful for those studying murine DC, but will also provide some useful ... information for those initiating human DC work. ... this updated book, which is unique in the field, will make a nice addition to personal and institutional biomedical research libraries." (Patricia Fitzgerald-Bocarsly, Doody's Review Service, July, 2010)