Cryopreservation and Freeze-Drying Protocols

Principles of Cryopreservation.- Principles of Cryopreservation By Vitrification.- Modeling and Optimization of Cryopreservation.- The Principles of Freeze-Drying.- Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezingand Drying of Cells.- Calorimetric Analysis Of Cryopreservation and Freeze-Drying Formulations.- Measurement Of Intracellular Ice Formation Kinetics By High-Speed Video Cryomicroscopy.- Laser Scanning Microscopy in Cryobiology.- Low Temperature Electron Microscopy.- Cryopreservation of Semen From Domestic Livestock.- Cryopreservation of Mammalian Oocytes.- Vitrification: A Simple And Successful Method for Cryostorage of Human Blastocysts.- Efficient Cryopreservation of Human Pluripotent Stem Cells By Surface-Based Vitrification.- Cryopreservation Of Greenshell(TM) Mussel (Perna Canaliculus) Sperm.- Membrane Modification Strategies for Cryopreservation.- Sperm Clean-Up and Density Centrifugation Procedures.- Cryopreservation of Red Blood Cells.- Cord Blood Clinical Processing, Cryopreservation, and Storage.- Directional Freezing For Large Volume Cryopreservation.- Vitrification Of Heart Valve Tissues.- Cryopreservation of Plant Cell Lines.- Writing Standard Operating Procedures (Sops) For Cryostorage Protocols: Using Shoot Meristem Cryopreservation As An Example.- Freeze-Drying Of Proteins.- Freeze-Drying Of Lactic Acid Bacteria.- Freeze-Drying Of Mammalian Sperm.- Freeze-Drying Of Decellularized Heart Valve Tissues.