Clinical Bioinformatics

In Silico Gene Discovery.- Whole Genome-Wide Association Study Using Affymetrix SNP Chip: A Two-Stage Sequential Selection Method to Identify Genes That Increase the Risk of Developing Complex Diseases.- Utilizing HapMap and Tagging SNPs.- Measuring the Effects of Genes and Environment on Complex Traits.- Microarrays-Planning Your Experiment.- Clinical Uses of Microarrays in Cancer Research.- Microarrays-Analysis of Signaling Pathways.- Microarrays-Identifying Molecular Portraits for Prostate Tumors with Different Gleason Patterns.- Microarrays for the Study of Viral Gene Expression During Human Cytomegalovirus Latent Infection.- Computer-Assisted Reading of DNA Sequences.- Evaluating DNA Sequence Variants of Unknown Biological Significance.- Developing a DNA Variant Database.- Protein Comparative Sequence Analysis and Computer Modeling.- Identification and Characterization of Microbial Proteins Using Peptide Mass Fingerprinting Strategies.- Statistical Analysis of Image Data Provided by Two-Dimensional Gel Electrophoresis for Discovery Proteomics.- Online Resources for the Molecular Contextualization of Disease.- Web-Based Resources for Clinical Bioinformatics.- Developing Decision Support Systems in Clinical Bioinformatics.- eConsulting.

In this volume of the Methods in Molecular Medicine, a number of strategies utilizing clinical bioinformatics are described. This series of articles focuses on software applications that can be used to translate information into outcomes of clinical relevance. Step-by-step instructions ensure successful results. The themes covered include gene discovery, gene function (microarrays), DNA mutation analysis, Proteomics, online approaches and resources, and Informatics in clinical practice. A challenge in clinical medicine is dealing with an ever increasing volume of information. This is particularly so in the emerging "omics" era, and impacts on researchers, health professionals and the broader community. To respond to this challenge requires computer-based storage, processing and dissemination i.e. bioinformatics.