Clinical Applications of Mass Spectrometry in Biomolecular Analysis
Methods and Protocols
(Sprache: Englisch)
This volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity,...
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Klappentext zu „Clinical Applications of Mass Spectrometry in Biomolecular Analysis “
This volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and no need for specialized reagents. The techniques described are a must for the measurement of many clinically relevant analytes in the fields of drug analysis, endocrinology, and inborn errors of metabolism. Each chapter provides a brief introduction about a specified analyte, followed by detailed instructions on the analytical protocol. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols is a great resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.
Inhaltsverzeichnis zu „Clinical Applications of Mass Spectrometry in Biomolecular Analysis “
Mass Spectrometry in Clinical Laboratory: Applications in Biomolecular Analysis.- Quantification of Free Carnitine and Acylcarnitines in Plasma or Serum Using HPLC/MS/MS.- Quantification of Arginine and its Methylated Derivatives in Plasma by High Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).- Quantitation of Albumin in Urine by Liquid Chromatography Tandem Mass Spectrometry.- Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography -Tandem Mass Spectrometry (LC-MS/MS).- Quantification of Five Clinically Important Amino Acids by HPLC-Triple TOF(TM) 5600 Based on Pre-Column Double Derivatization Method.- Sensitive, Simple, and Robust Nano Liquid Chromatography-Mass Spectrometry Method for Amyloid Protein Subtyping.- Quantitation of Ubiquinone (Coenzyme Q10) in Serum/Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (ESI-LC-MS/MS).- Quantitative Analysis of Salivary Cortisol Using LC-MS/MS.- Quantification of Dihydroxyacetone Phosphate (DHAP) in Human Red Blood Cells by HPLC-TripleTOF 5600(TM) Mass Spectrometer.- Simultaneous Quantitation of Estradiol and Estrone in Serum Using Liquid Chromatography Mass Spectrometry.- Direct Measurement of Free Estradiol in Human Serum and Plasma by Equilibrium Dialysis-Liquid Chromatography-Tandem Mass Spectrometry.- Quantification of Gamma-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry.- Quantitation of Insulin Analogues in Serum Using Immunoaffinity Extraction, Liquid Chromatography, and Tandem Mass Spectrometry.- Quantitation of Insulin Like Growth Factor 1 in Serum by Liquid Chromatography High Resolution Accurate-Mass Mass Spectrometry.- Quantitation of Free Metanephrines in Plasma by Liquid Chromatography Tandem Mass Spectrometry.- Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography Tandem Mass Spectrometry.- High Throughput Analysis of Methylmalonic Acid in Serum,
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Plasma and Urine by LC-MS/MS: Method for Analyzing Isomers without Chromatographic Separation.- Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry.- Quantitative Organic Acids in Urine by Two Dimensional Gas Chromatography Time of Flight Mass Spectrometry (GCxGC-TOFMS).- High Sensitivity Measurement of Pancreatic Polypeptide and its Variant in Serum and Plasma by LC-MS/MS.- Quantitation of Parathyroid Hormone in Serum or Plasma by Liquid Chromatography Tandem Mass Spectrometry.- Determination of Phenylalanine and Tyrosine by High Performance Liquid Chromatography Tandem Mass Spectrometry.- Urine Purine Metabolite Determination by UPLC Tandem Mass Spectrometry.- Urine Pyrimidine Metabolite Determination by HPLC Tandem Mass Spectrometry.- Quantitation of Plasma Renin Activity in Plasma Using Liquid Chromatography -Tandem Mass Spectrometry (LC-MS/MS).- Quantitation of S-Adenosylmethionine and S-Adenosylhomocysteine in Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry.- A Simple, High Throughput Method for Analysis of Ceramide, Glucosylceramide, and Ceramide Trihexoside in Dried Blood Spots by LC/MS/MS.- Quantification of Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone and Testosterone by Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS).- Urinary Succinylacetone Analysis by Gas Chromatography-Mass Spectrometry (GC-MS).- Quantification of 1,25-Dihydroxyvitamin D2 & D3 in Serum Using Liquid Chromatography Tandem Mass Spectrometry.- High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation.- Quantitation of 25-OH-Vitamin-D2 and 25-OH-Vitamin-D3 in Urine Using LC-MS/MS.
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Bibliographische Angaben
- 2016, Softcover reprint of the original 1st ed. 2016, XV, 333 Seiten, Masse: 17,8 x 25,4 cm, Kartoniert (TB), Englisch
- Herausgegeben: Uttam Garg
- Verlag: Springer, Berlin
- ISBN-10: 1493950096
- ISBN-13: 9781493950096
Sprache:
Englisch
Pressezitat
"This is a compendium of mass spectrometry protocols for clinically important analytes. ... This book is intended for anyone interested in clinical applications of mass spectrometry. It would be of interest to basic scientists, clinical laboratory practitioners (e.g., scientists, physicians, etc.), manufacturers, and healthcare providers interested in applications of mass spectrometry. ... This is your book if you are contemplating implementing mass spectrometry in your laboratory for any of these clinically important analytes." (Valerie L. Ng, Doody's Book Reviews, March, 2016)
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