Cereal Genomics
Methods and Protocols
(Sprache: Englisch)
?In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes. Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more...
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Produktinformationen zu „Cereal Genomics “
?In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes. Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described. Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.
Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.
Klappentext zu „Cereal Genomics “
In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes. Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described. Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.
Inhaltsverzeichnis zu „Cereal Genomics “
1. Dna Extraction from Vegetative Tissue for Next Generation Sequencing Agnelo Furtado2. DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples), Chiaki Mutou, Katsunori Tanaka, Ryuji Ishikawa
3. RNA Extraction from Cereal Vegetative Tissue Julie A. Pattemore
4. RNA Extraction from Developing or Mature Wheat Seeds Agnelo Furtado
5. cDNA Library Preparation Maarten Kooiker and Gang-Ping Xue
6. Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants Siddanagouda S. Biradar, Xiaojun Nie, Kewei Feng and Song Weining
7. The Polymerase Chain Reaction (PCR) - General Methods Daniel L. E. Waters and Frances M. Shapter
8. Mutation and Mutation Screening L. Slade Lee, Bradley J. Till,, Helen Hill, Owen A. Huynh, and Joanna Jankowicz-Cieslak
9. The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression Timothy L. Fitzgerald and Richard B. McQualter
10. Cloning of DNA Fragments: Ligation Reactions in Agarose Gel, Agnelo Furtado
11. Rapid Cloning of Genes and Promoters for Functional Analyses Peer M. Schenk
12. Genome Walking Frances M. Shapter and Daniel L.E. Waters
13. Functional Analysis by Protein Biochemistry Louis M. T. Bradbury
14. Genomic Southern Blot Analysis Leigh Gebbie
15. Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs, Shazia Iram
16. Protein Blotting-Protocol for Beginners Lars A. Petrasovits
17. Genetic Transformation of Wheat via Particle Bombardment Caroline A. Sparks & Huw D. Jones
18. Sorghum Genetic Transformation by Particle Bombardment, Guoquan Liu, Bradley C. Campbell and Ian D. Godwin
19. Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery Caroline A. Sparks, Angela Doherty & Huw D. Jones
20. A Protocol for High-throughput Agrobacterium-mediated Barley Transformation, Wendy A Harwood
21. Agrobacterium-mediated Transformation- Rice
... mehr
Transformation Inez H. Slamet-Loedin, Prabhjit Chadha-Mohanty, Lina Torrizo
22. Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos Hyeyoung Lee and Zhanyuan J. Zhang
23. A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops Zining Wang
24. A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data Gopala Krishnan, S., Daniel L. E. Waters and Robert J. Henry
22. Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos Hyeyoung Lee and Zhanyuan J. Zhang
23. A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops Zining Wang
24. A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data Gopala Krishnan, S., Daniel L. E. Waters and Robert J. Henry
... weniger
Bibliographische Angaben
- 2013, Repr. d. Ausg. v. 2013, X, 300 Seiten, 29 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Robert J. Henry, Agnelo Furtado
- Verlag: Springer, Berlin
- ISBN-10: 1627037144
- ISBN-13: 9781627037143
- Erscheinungsdatum: 25.11.2013
Sprache:
Englisch
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