Cell Surface Receptors

- Introduction to Receptor Theory
... Origin of the Receptor Concept
... Occupancy Theory -Relationship Between Occupancy and Response
... Concept of Spare Receptors
... Operational Models of Pharmacological Agonism
... Rate Theory
... Allosteric Theory
... Beyond Two-State Receptor Theory
... Summary.

- Characterization of Receptors Based on Receptor-Mediated Responses
... Characterization of Receptor Specificity
... Determining Equilibrium Dissociation Constants (Kd Values) for Receptor-Ligand Interactions Based on Measurements of Receptor-Mediated Response
... Pharmacologic Resultant Analysis
... Summary.

- Identification of Receptors Using Direct Radioligand Binding Techniques
... Methods
... Data Generation
... Criteria Expected for Binding of *D to the Physiologically Relevant Receptor, R
... Determination of Rate Constants for Radioligand Association and Dissociation
... Summary.

- Complex Binding Phenomena
... Mathematical Descriptions of Complex Binding Phenomena 124
... Non-Linear Regression Analysis of Complex Binding Phenomena
... Independent Receptor Subtypes
... Affinity States of a Single Receptor Population
... Summary.

- The Preparation and Study of Detergent-Solubilized Receptors
... General Properties of Biological Membranes and Detergent Micelles
... Choice of a Biological Detergent
... Solubilizing Receptors from Biological Membranes
... Methods for Analysis of Detergent-Solubilized Receptors
... Summary.

- Quantifying Cell Surface Receptor Binding and Turnover in Intact Cells
... Discriminating Between Cell Surface Versus Intracellular Receptor-Ligand Complexes
... Biochemical Evidence Consistent with Recycling of Cell Surface Receptors
... Assessment of Rate Constants for Receptor Turnover Using a Steady State Mathematical Analysis of Intact Cell Radioligand Binding Data
... The Heavy Amino Acid Density-Shift Technique for Quantitating Receptor Synthesis and Turnover
... Summary.

- Index