Caveolae

Selective Visualization of Caveolae by TEM using APEX2.- Freeze-Fracture Replica Immunolabeling of Cryopreserved Membrane Compartments, Cultured Cells and Tissues.- Analysis of Caveolin in Primary Cilia.- Method for Efficient Observation of Caveolin-1 Plasma Membrane by Microscopy Imaging Analysis.- Quantitative Image Analysis of the Spatial Organization and Mobility of Caveolin Aggregates at the Plasma Membrane.- Spatiotemporal Analysis of Caveolae Dynamics using Total Internal Reflection Fluorescence Microscopy.- Live-Cell FRET Imaging of Phosphorylation-Dependent Caveolin-1 Switch.- GPMVs as a Tool to Study Caveolin-Interacting Partners.- Biotin Proximity Labeling to Identify Protein-Protein Interactions for Cavin1.- Investigation of Novel Cavin-1/Suppressor of Cytokine 3 (SOCS3) Interactions by Co-Immunoprecipitation, Peptide Pull-Down and Peptide Array Overlay Approaches.- Analysis of Protein and Lipid Interactions using Liposome Co-Sedimentation Assays.- Liposome Binding Assay to Characterize Cavin Family Protein Structure and Functions.- Preparation of Caveolin-1 for NMR Spectroscopy Experiments.- Tagging and Deleting of Endogenous Caveolar Components using CRISPR/Cas9 Technology.- Pulling of Tethers from the Cell Plasma Membrane using Optical Tweezers.- Live Confocal Imaging of Zebrafish Notochord Cells under Mechanical Stress In Vivo.- Study of Caveolae Dependent Mechanoprotection in Huma Muscle Cells using Micropatterning and Live Cell Microscopy.- Immunofluorescence-Based Analysis of Caveolin-3 in the Diagnostic Management of Neuromuscular Diseases.