ATM Kinase

Assaying Radiosensitivity of Ataxia-Telangiectasia.- Assaying for Radioresistant DNA Synthesis, the Hallmark Feature of the Intra-S-Phase Checkpoint Using a DNA Fiber Fluorography-Based Methodology.- ATM Gene Mutation Detection Techniques and Functional Analysis.- An HTRF® Assay for the Protein Kinase ATM.- ATM Kinase Inhibitors: HTS Cellular Imaging Assay Using CellomicsTM ArrayScan VTI Platform.- Image-Based High Content Screening: Automating the Quantification Process for DNA Damage-Induced Foci.- Analyzing ATM Function by Electroporation of Endonucleases and Immunofluorescence Microscopy.- Quantitative and Dynamic Imaging of ATM Kinase Activity by Bioluminescence Imaging.- Zn(II)-Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein.- Identification of ATM Protein Kinase Phosphorylation Sites by Mass Spectrometry.- Studies of ATM Kinase Activity Using Engineered ATM Sensitive to ATP Analogues (ATM-AS).- Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies.- Identification of ATM-Interacting Proteins by Co-Immunoprecipitation and Glutathione-S-Transferase (GST) Pull-Down Assays.- ATM Activation and H2AX Phosphorylation Induced by Genotoxic Agents Assessed by Flow- and Laser Scanning-Cytometry.- Peptide Immunoaffinity Enrichment with Targeted Mass Spectrometry: Application to Quantification of ATM Kinase Phospho-Signaling.- Mass Spectrometry-Based Proteomics for Quantifying DNA Damage-Induced Phosphorylation.- Statistical Analysis of ATM-Dependent Signaling in Quantitative Mass Spectrometry Phosphoproteomics.- ChIP Technique to Study Protein Dynamics at Defined DNA Double-Strand Breaks.- Studies of the DNA Damage Response by Using the Lac Operator/Repressor (LacO/LacR) Tethering System.- Imaging of Fluorescently-Tagged ATM Kinase at the Sites of DNA Double-Strand Breaks.- Live Cell