Arbuscular Mycorrhizal Fungi

Isolation and Culture of Arbuscular Mycorrhizal Fungi from Field Samples.- The Mycorrhizal Donor Plant (MDP) In Vitro Culture System for the Efficient Colonization of Whole Plants.- A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium.- Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization.- Fluorescent Staining of Arbuscular Mycorrhizal Structures Using Wheat Germ Agglutinin (WGA) and Propidium Iodide.- Visualization of Arbuscular Mycorrhizal Fungal Extra-Radical Hyphae and Spores Vitality and Activity.- Molecular Quantification of Arbuscular Mycorrhizal Fungal Root Colonization.- Single-Spore-Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi.- High Throughput DNA Sequence-Based Analysis of AMF Communities.- Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction Fragment Length Polymorphism (TRFLP).- Bioinformatic Methods for the Analysis of High-Throughput RNA Sequencingin Arbuscular Mycorrhizal Fungi.- Arbuscular Mycorrhizal Fungal Gene Expression Analysis by Real-Time PCR.- Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis.- Detection of Arbuscular Mycorrhizal Fungal Gene Expression by In Situ Hybridization.- Functional Analyses of Arbuscular Mycorrhizal Fungal Genes in Yeast.- Isotope Labelling to Study Phosphorus Uptake in the Arbuscular Mycorrhizal Symbiosis.- Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae.- Host-Induced Gene Silencing of Arbuscular Mycorrhizal Fungal Genes via Agrobacterium Rhizogenes-Mediated Root Transformation in Medicago truncatula.- Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi.