Antigen Processing

Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes.- Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry.- Trimming of MHC Class I Ligands by ERAP Aminopeptidases.- Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases.- Testing the Impact of Protease Inhibitors in Antigen Presentation Assays.- In Vitro Studies of MHC Class I Peptide Loading and Exchange.- Measuring Synthesis and Degradation of MHC Class I Molecules.- Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules.- A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics.- Purification and Identification of Naturally Presented MHC Class I and II Ligands.- Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs.- Quantitating MHC Class I Ligand Production and Presentation using TCR-LikeAntibodies.- Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors.- A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway.- Evaluating CD8+ T Cell Responses In Vitro.- Recombinant Poxviruses: Versatile Tools for Immunological Assays.- Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy.- Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases.- Monitoring MHC-II Endocytosis and Recycling using Cell-Surface Protein Biotinylation-Based Assays.- Monitoring Protein Endocytosis and Recycling using FACS-Based Assays.- In Vitro Digestion with Proteases Producing MHC Class II Ligands.- Assembly, Intracellular Transport and Release of MHC Class II Peptide Receptors.- Pulse-Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading.- Studying MHC Class II Peptide Loading andEditing