Adenovirus Methods and Protocols

Ann E. Tollefson, Mohan Kuppuswamy, and William S. M. WoldTABLE OF CONTENTSVol. II Topics: Ad proteins and RNA, lifecycle and host interactions, and phylogenetics1. Analysis of the Efficiency of Adenovirus TranscriptionCristina Iftode and S. J. Flint2. The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein DomainsPaul M. Loewenstein, Chao-Zhong Song and Maurice Green3. Preparation of Soluble Extracts from Adenovirus Infected Cells for Studies of RNA SplicingOliver Mühlemann and Göran Akusjärvi4. In Vitro Methods to Study RNA Interference During an Adenovirus InfectionGunnar Andersson, Ning Xu and Göran Akusjärvi5. Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In SituEileen Bridge6. Study of Nucleolar Localization of Adenovirus Core ProteinsDavid A. Matthews7. Analysis of Adenovirus Infections in Synchronized CellsDavid A. Ornelles, Robin N. Shepard and Felicia D. Goodrum8. Co-Immunoprecipitation of Protein ComplexesPeter Yaciuk9. Chromatin Immunoprecipitation (ChIP) to Study the Binding of Proteins to the Adenovirus Genome in VivoJihong Yang and Patrick Hearing10. Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift AssaysPilar Perez-Romero and Michael J. Imperiale11. Identifying Functional Adenovirus-Host Interactions Using Tandem Mass SpectrometryAnuj Gaggar, Dmitry Shayakhmetov, and Andre Lieber12. The Use of Cell Microinjection for the in vivo Analysis of Viral Transcriptional Regulatory Protein DomainsMaurice Green, Andrew Thorburn, Robert Kern and Paul M. Loewenstein13. Determination of the Transforming Activities of Adenovirus OncogenesMichael Nevels and Thomas Dobner14. Human Adenovirus Type 12: Crossing Species Barriers toImmortalize the Viral GenomeWalter Doerfler15. Measurement of NK Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed CellsJohn M. Routes16. A Flow Cytometric Assay for Analysis of NK

"...recommend it to investigators working in the area of viral-host interactions." -Doody's Book Review, Weighted Numerical Score: 70 - 3 Stars Reviews of the First edition:"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."- Microbiology Today". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters