Protein-Ligand Interactions (PDF)

Frontmatter -- Preface -- Section I. General -- Letter from J. Wyman to the Members of the Symposium -- Concerted Changes in an Allosteric Macromolecule -- Ligand Interactions in Globular Proteins -- Discussion -- Ligand Binding to Enzyme Complexes -- Discussion -- Properties of Polymeric Proteins Bound to Solid Matrices: Subunit Exchange Chromatography -- Discussion -- Contributions of Aromatic Amino Acid Residues to the Optical Activity of Protein-Ligand Complexes -- Discussion -- Section II. Enzymes -- The Architecture of the Coenzyme Binding Domain in Dehydrogenases as Revealed by X-Ray Structure Analysis -- Discussion -- The Interaction of Glutamate Dehydrogenase with a Coenzyme Analog -- Discussion -- The Interaction of Adenosine Triphosphate and its Analogs with Myosin and the Modification by Actin -- Discussion -- Isolation of a Guanylnucleotide Binding Protein from Pigeon Erythrocyte Membranes -- Discussion -- On the Binding of Isoleucine and Related Amino Acids to Isoleucyl-tRNA Synthetase from Escherichia Coli MRE 600 -- Discussion -- The Effect of Small Molecules on the Affinity of Individual Chains in Hemoglobin and on its Cooperativity -- Discussion -- Cooperative Interactions without Symmetry or Subunits -- Discussion -- A Nuclear Magnetic Resonance Study of Activator Binding by Carboxypeptidase A -- Discussion -- Section III. Repressors -- Lactose Operator Sequences and the Action of Lac Repressor -- Discussion -- The Active Sites of Lac Repressor -- Discussion -- Lac Repressor -- Discussion -- The Use of Suppressed Nonsense Mutations to Generate Altered Lac Repressor Molecules -- Discussion -- Effect of Alterations in Lac Operator DNA on Repressor Binding -- Discussion -- Interaction of Lac Repressor with Non-Specific DNA Binding Sites -- Discussion -- Section IV. Receptors -- Binding and Functional States of the Cholinergic Receptor Protein from Torpedo Marmorata -- Discussion -- Subunit Structure and Binding Sites of the Acetylcholine Receptor -- Discussion -- Further Characterization of Purified Acetylcholine Receptor and its Incorporation into Phospholipid Vesicles -- Discussion -- Structural and Functional Studies of an Acetylcholine Receptor -- Discussion -- Section V. Antibodies, Drugs and Metabolites -- Antibody Combining Sites as a Model for Molecular Recognition -- Discussion -- Antibiotic Action on Enzymes Involved in Peptidoglycan Synthesis -- Discussion -- Problems in Studying Protein Binding with Regard to the Interaction of Steroids with a Specific Binding Globulin -- Discussion -- The Interaction of Low-Molecular Compounds with Serum Albumin as Measured by Optical Methods. Circular Dichroism of Bilirubin-Human Serum Albumin Complexes in the Presence of Alcohols in Aqueous Solution -- Discussion -- Section VI. Detergents and Electrolytes -- The Nature of Specific and Non-Specific Interactions of Detergents with Proteins: Complexing and Unfolding -- Discussion -- Interaction of Detergents with Proteins: Effect of Counter Ions on the System Ferrimyoglobin- Laurylpyridinium Halides in Aqueous Medium -- Discussion -- Influence of Ions on the Kinetics of Cooperative Conformational Changes in Globular Proteins -- Discussion -- Neutral Salt Effects on the Conformational Stability of Biological Macromolecules -- Discussion -- Concluding Remarks -- Index of Contributors -- Subject Index -- Backmatter