Sirtuins
Methods and Protocols
(Sprache: Englisch)
Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes. Opening with methods to generate sirtuin biology tools,...
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Klappentext zu „Sirtuins “
Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes. Opening with methods to generate sirtuin biology tools, the book continues by covering methods to identify sirtuin substrates, to measure sirtuin activity, and to study sirtuin biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and easy to use, Sirtuins: Methods and Protocols presents detailed protocols for sirtuin research that can be followed directly or modified to investigate new areas of sirtuin biology.
Inhaltsverzeichnis zu „Sirtuins “
Part I: Methods to Generate Sirtuin Biology Tools1. Introduction: Sirtuins in Aging and Diseases
Leonard Guarente
2. Sirtuins in Yeast: Phenotypes and Tools
Scott Tsuchiyama, Elizabeth Kwan, Weiwei Dang, Antonio Bedalov, and Brian K. Kennedy
3. Studying the C. elegans Sirtuins
Mohan Viswanathan and Heidi A. Tissenbaum
4. Genetic and Biochemical Tools for Investigating Sirtuin Function in Drosophila melanogaster
Jason G. Wood, Rachel Whitaker, and Stephen L. Helfand
5. Generating Mammalian Sirtuin Tools for Protein-Interaction Analysis
Kathleen A. Hershberger, Jonathan Motley, Matthew D. Hirschey, and Kristin A. Anderson
Part II: Methods to Identify Sirtuin Substrates
6. Mass Spectrometry-Based Detection of Protein Acetylation
Yu Li, Jeffrey C. Silva, Mary E. Skinner, and David B. Lombard
7. SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome
Yue Chen, Gozde Colak, and Yingming Zhao
8. Targeted Quantitation of Acetylated Lysine Peptides by Selected Reaction Monitoring Mass Spectrometry
Matthew J. Rardin, Jason M. Held, and Bradford W. Gibson
9. Identification of Deacetylase Substrates with the Biotin Switch Approach
J. Will Thompson, Alex Robeson, and Joshua L. Andersen
10. Assaying Chromatin Sirtuins
Lei Zhong, Barbara Martinez-Pastor, Dafne M. Silberman, Carlos Sebastian, and Raul Mostoslavsky
Part III: Methods to Measure Sirtuin Activity
11. Measurement of Sirtuin Enzyme Activity Using a Substrate-Agnostic Fluorometric Nicotinamide Assay
Basil P. Hubbard and David A. Sinclair
12. Detecting Sirtuin-Catalyzed Deacylation Reactions Using 32 P-Labeled NAD and Thin-Layer Chromatography
Anita Zhu, Xiaoyang Su, and Hening Lin
13. Chemical Acetylation And Deacetylation
Kristofer S. Fritz
14. Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD + ) with High-Performance Liquid Chromatography
Jun Yoshino and Shin-ichiro Imai
15. In Vivo Measurement of the
... mehr
Acetylation State of Sirtuin Substrates as a Proxy for Sirtuin Activity
John Dominy, Pere Puigserver, and Carles Cantó
Part IV: Methods to Study Sirtuin Biology
16. Oxygen Flux Analysis to Understand the Biological Function of Sirtuins
Dongning Wang, Michelle F. Green, Eoin McDonnell, and Matthew D. Hirschey
17. The Emerging Links Between Sirtuins and Autophagy
In Hye Lee, Jeanho Yun, and Toren Finkel
18. Methods to Study the Role of Sirtuins in Genome Stability
Paloma Martínez-Redondo and Alejandro Vaquero
19. Circadian Measurements of Sirtuin Biology
Kathryn Moynihan Ramsey, Alison H. Affinati, Clara B. Peek, Biliana Marcheva, Hee-Kyung Hong, and Joseph Bass
20. Utilizing Calorie Restriction to Evaluate the Role of Sirtuins in Healthspan and Lifespan of Mice
Jessica Curtis and Rafael de Cabo
John Dominy, Pere Puigserver, and Carles Cantó
Part IV: Methods to Study Sirtuin Biology
16. Oxygen Flux Analysis to Understand the Biological Function of Sirtuins
Dongning Wang, Michelle F. Green, Eoin McDonnell, and Matthew D. Hirschey
17. The Emerging Links Between Sirtuins and Autophagy
In Hye Lee, Jeanho Yun, and Toren Finkel
18. Methods to Study the Role of Sirtuins in Genome Stability
Paloma Martínez-Redondo and Alejandro Vaquero
19. Circadian Measurements of Sirtuin Biology
Kathryn Moynihan Ramsey, Alison H. Affinati, Clara B. Peek, Biliana Marcheva, Hee-Kyung Hong, and Joseph Bass
20. Utilizing Calorie Restriction to Evaluate the Role of Sirtuins in Healthspan and Lifespan of Mice
Jessica Curtis and Rafael de Cabo
... weniger
Bibliographische Angaben
- 2013, 314 Seiten, 36 farbige Abbildungen, 33 Schwarz-Weiss-Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben von Hirschey, Matthew D.
- Herausgegeben: Matthew D. Hirschey
- Verlag: Humana Press
- ISBN-10: 1627036369
- ISBN-13: 9781627036368
- Erscheinungsdatum: 07.09.2013
Sprache:
Englisch
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