Quantitative Proteomics by Mass Spectrometry

Quantitative Proteomics by Mass Spectrometry Editor Salvatore Sechi Table of Contents 1. Preface by Salvatore Sechi: An Overview of Quantitative Proteomics by Mass Spectrometry. 2. Illarion V. Turko and Salvatore Sechi: Acrylamide - A Cysteine Alkylating Reagent for Quantitative Proteomics. 3. Jeffrey A. Ranish, Marjorie Brand and Ruedi Aebersold: Using stable isotope tagging and MS to characterize protein complexes and to detect changes in their composition. 4. Shao-En Ong and Matthias Mann: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics. 5. Toshitaka Sato, Yasushi Ishihama, Yoshiya Oda: Quantitative Proteomics of Mouse Brain, and Specific Protein-Interaction Studies using Stable Isotope Labeling. 6. Scott A. Gerber, Arminja N. Kettenbach, John Rush and Steven P. Gygi: The Absolute Quantification Strategy: Application to Phosphorylation Profiling of Human Separase Serine 1126. 7. Sushmita Mimi Roy and Christopher H. Becker: Quantification of proteins and metabolites by mass spectrometry without isotopic labeling. 8. Tao Liu, Wei-Jun Qian, David G. Camp, II, and Richard D. Smith: The Use of a Quantitative Cysteinyl-peptide Enrichment Technology for High-Throughput Quantitative Proteomics. 9. Fred E. Regnier: An isotope coding strategy for proteomics involving both amine and carboxyl group labeling 10. Catherine Fenselau and Xudong Yao: Proteolytic Labeling with 18O for Comparative Proteomics Studies: preparation of 18O-labeled peptides and the 18O/16O peptide mixture 11. Franti_ek Ture_ek, C. Ronald Scott, and Michael H. Gelb: Tandem mass spectrometry in the detection of inborn errors of metabolism and newborn screening. 12. Yu Lu, Patricia Bottari, Ruedi Aebersold, Franti_ek Ture_ek, and Michael H. Gelb: Absolute quantitation of proteins in complex mixtures by the Visible Isotope-Coded Affinity Tagsmethod. 13. Michael J. MacCoss and Christine C. Wu.: Computational Analysis of Quantitative Proteomics Data using Stable

From the reviews: "Quantitative Proteomics by Mass Spectrometry describes protocols of various quantitative proteomics procedures utilizing mass spectrometry. ... The publication of this book is timely. ... provides useful information regarding sample preparation/handling, reagent preparation/handling, mass spectrometry analysis etc. for successful experiments. ... In summary, the book serves as an excellent guide for both the experienced investigator and novice in the field who is contemplating using quantitative proteomics by mass spectrometry."­­­ (Masaru Miyagi, Journal of the American Society for Mass Spectrometry, Vol. 18, 2007)