Protein Targeting Protocols

- Contents

- Preface
- Contributors

- Bacterial systems
1. Localisation and mobility of bacterial proteins by confocal microscopy and fluorescence recovery after photobleaching (FRAP)
- Conrad Mullineaux
2. Membrane protein insertion and secretion in bacteria
- Jeanine de Keyzer, Martin van der Laan and Arnold J.M. Driessen
3. Identification of autotransporter proteins secreted by type V secretion systems in Gram-negative bacteria
- Yihfen T. Yen and Christos Stathopoulos
4. Periplasmic targeting of recombinant proteins in Escherichia coli
- Filipe J. M. Mergulhão and Gabriel A. Monteiro
5. In vitro analysis of the bacterial twin-arginine-dependent protein export
- Michael Moser, Sascha Panahandeh, Eva Holzapfel and Matthias Müller

- Eukaryotic systems
6. Nuclear import properties of the sex determining factor SRY
- Jade K. Forwood, Gurpreet Kaur and David A. Jans
7. The mitochondrial machinery for import of precursor protein, a review.
- Kip Gabriel and Nikolaus Pfanner
8. A genetic method to identify mitochondrial proteins in living mammalian cells
- Takeaki Ozawa and Yoshio Umezawa
9. In vitro and in vivo methods to study protein import into plant mitochondria
- Shashi Bhushan, Pavel F. Pavlov, Charlotta Rudhe and Elzbieta Glaser
10. Protein targeting to mitochondria of Saccharomyces cerevisiae and Neurospora crassa; in vitro and in vivo studies
- Panagiotis Paptheodorou, Gra_nya Doma_ska and Joachim Rassow
11. A mitosome purification protocol based on Percoll density gradients and its use in validating the mitosomal nature of Entamoeba histolytica mitochondrial Hsp70
- Jorge Tovar, Siân S.E. Cox and Mark van der Giezen
12. The chloroplast protein import machinery, a review
- Friederike Hörmann, Jürgen Soll and Bettina Bölter
13. Import of plastid precursor proteins into pea chloroplasts
- Jürgen Soll and Bettina Bölter
14. Protein targeting in "secondary" or "complex" chloroplasts
- Balbir K. Chaal and Beverley R. Green
15.