Parallel Single Cell Electrophoresis in a Microchip Platform
(Sprache: Englisch)
Single cell analysis has typically been difficult and challenging to perform in such a way that meaningful statistical information can be extracted. This thesis documents the development of a microchip or lab-on-a-chip platform to perform single cell...
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Single cell analysis has typically been difficult and challenging to perform in such a way that meaningful statistical information can be extracted. This thesis documents the development of a microchip or lab-on-a-chip platform to perform single cell electrophoresis in parallel separation channels. It describes the use of optical tweezers to select a cell and transport it to a desired separation channel. It also describes the use of electromechanical shearing to lyse a cell. These two techniques are combined with laser-micromachined separation channels to perform simple electrohoresis on the fluorescently labeled cellular contents.
Bibliographische Angaben
- Autoren: Nigel Munce , Lothar Lilge
- 2010, 140 Seiten, Masse: 22 cm, Kartoniert (TB), Englisch
- Verlag: LAP Lambert Academic Publishing
- ISBN-10: 3838304489
- ISBN-13: 9783838304489
Sprache:
Englisch
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