Nuclear Reprogramming

Nuclear Transfer in the Mouse.- Nuclear Transfer in the Rabbit.- Nuclear Transfer in Ruminants.- Nuclear Transfer and Transgenesis in the Pig.- Embryonic Stem Cell-Somatic Cell Fusion and Post-Fusion Enucleation.- Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte.- Assessing the Quality of Donor Cells: Karyotyping Methods.- Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer.- Fluorescent Immunodetection of Epigenetic Modifications on Pre-Implantation Mouse Embryos.- Visualization of Epigenetic Modifications in Preimplantation Embryos.- Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer.- Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming.- Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining.- Gene Expression Analysis in Early Embryos through Reverse Transcription Quantitative PCR (RT-qPCR).- Studying Bovine Early Embryo Transcriptome by Microarray.- Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level.- Micro Chromatin Immunoprecipitation ( ChIP) from Early Mammalian Embryos.- Assessing Reprogramming by Chimera Formation and Tetraploid Complementation.- Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology.- Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP).