Nuclear Receptors

Nuclear Receptors: A Historical Perspective.- An Optimized Immunoblotting Protocol for Accurate Detection of Endogenous PGC-1 Isoforms in Various Rodent Tissues.- Sub-Cellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue.- In Vivo Quantitation of Estrogen Receptor b Subtype Expression in Ovarian Surface Epithelium Using Immunofluorescence Profiling and Confocal Microscopy.- In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High Confidence Genome-Wide Binding Profiles.- High-Content Analysis of Constitutive Androstane Receptor Nuclear Translocation.- Antibody Validation Strategy for Nuclear Receptors.- Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed Paraffin-Embedded Tissue.- Detection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 from Archaeoglobus fulgidus.- Reconstitution of the Steroid ReceptorHeterocomplex.- High-Throughput Imaging of PPIX Using Confocal Microscopy.- PGC-1 Overexpression via Local In Vivo Transfection in Mouse Skeletal Muscle.- A Reverse Transfection Method for Screening of Nuclear Receptor Activators.- Hybrid Reporter Gene Assays: Versatile In Vitro Tools to Characterize Nuclear Receptor Modulators.- Analysis of the Transcriptional Activity of Retinoic Acid-Related Orphan Receptors (RORs) and Inhibition by Inverse Agonists.- Examining the Role of Nuclear Receptors during In Vivo Chemical-Mediated Breast Tumorigenesis.- Analysis of IL-4/STAT6 Signaling in Macrophages.- Isolation and Characterization of Adipose Tissue Macrophages.- Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph.- Generation of Skeletal Myocytes from Embryonic Stem Cells through Nuclear Receptor Signaling.- Obtaining Crystals of PPARGamma Ligand Binding Domain Bound to Small Molecules.- Molecular Modeling Approach to Study the PPARGamma-Ligand