Modern Fluorescence Spectroscopy

1. Structural Interpretation of Fluorescence Spectra by Automated File Searching. Implementation and Applications in Liquid Chromatography.- A. Introduction.- B. Alternatives for Computerized Spectral Interpretation.- C. An Initial Approach.- D. Using More Subtle Features.- E. Limitations.- F. Hardware for an LC/Fluorescence System.- G. Reverse Searching.- H. Performance and Outlook.- References.- 2. Fluorescence Detection in Liquid and Gas Chromatography: Techniques, Examples, and Prospects.- A. Introduction.- B. Detection of Compounds That Exhibit Significant Native Fluorescence.- C. Detection of Compounds That Do Not Exhibit a Significant Fluorescence Quantum Yield.- D. Further Instrumental Considerations in HPLC Detection by Fluorescence.- E. Future Trends in Fluorometric HPLC Detection.- F. Fluorescence Detection in Gas Chromatography.- References.- 3. Reaction Rate Methods in Fluorescence Analysis.- A. Introduction.- B. Characteristics of Fluorescence Kinetic Measurements.- C. Applications of Fluorometric Kinetic Methods.- D. Instrumentation for Fluorescence Rate Measurements.- E. Conclusions.- References.- 4. Principles and Practice of Fluoroimmunoassay Procedures.- A. Introduction.- B. Principles of Immunoassay.- C. Fluoroimmunoassay Reagents and Equipment.- D. Fluoroimmunoassay Techniques.- E. Fluoroimmunoassay Limitations and Interfering Factors.- F. Selected Examples of Fluoroimmunoassay Development and Application.- G. Future Trends and Developments.- H. Summary.- I. Appendix: Glossary of Terms.- References.- 5. Fluorometric Studies of Biologically Important Molecular Complexes.- A. Introduction.- B. Complexes of Acridine Drugs with Nucleotides and DNA.- C. Complexes Involving Hydrogen Bonds.- D. Complexes between Oligopeptides and Proteins with Nucleic Acids.- E. Dynamics of Membranes Probed by Fluorophores Forming Complexes.- F. Intrinsic and Extrinsic Fluorescent Probes of Biomolecules.- References.- 6. Fluorometric Quantification of Specific Chemical