Metabolic engineering of Basfia succiniciproducens for the production of carbon-three compounds (Band 7)
(Sprache: Englisch)
Beschreibung The recent rise of bio-succinic acid as an industrial platform chemical has brought the rumen bacterium Basfia succiniciproducens, a natural producer of this organic acid, into the focus of research. This work investigated the potential of B....
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Beschreibung The recent rise of bio-succinic acid as an industrial platform chemical has brought the rumen bacterium Basfia succiniciproducens, a natural producer of this organic acid, into the focus of research. This work investigated the potential of B. succiniciproducens for the production of carbon-three chemicals from sugars. Beside the optimization of improved genetic tools, the C3 chemicals alanine, ¿-alanine and 3-hydroxypropionate (3-HP) were targeted. A new alanine producer, B. succiniciproducens ALA-1, exhibited good performance in the bioprocess. The next step demonstrated the proof-of-concept for ¿-alanine synthesis using the heterologous expression of aspartate 1 decarboxylase genes from Corynebacterium glutamicum or Vibrio natriegens. The expression of two additional genes enabled the de novo synthesis of up to 100 mg L-1 3-HP from glucose.
Bibliographische Angaben
- Autor: Jonathan Thomas Fabarius
- 2018, 144 Seiten, Masse: 14,8 x 21 cm, Kartoniert (TB), Englisch
- Verlag: Cuvillier Verlag
- ISBN-10: 3736999135
- ISBN-13: 9783736999138
- Erscheinungsdatum: 06.12.2018
Sprache:
Englisch
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