Mass Spectrometry of Proteins and Peptides
Methods and Protocols
(Sprache: Englisch)
When the last edition of this book was published in 2000, the field of proteomics was in its infancy. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to...
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When the last edition of this book was published in 2000, the field of proteomics was in its infancy. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. As a result of these rapid developments that have led to expanded areas of research and innovative applications, an update to Mass Spectrometry of Proteins and Peptides is not only worthwhile, but arguably mandatory. Spanning fields from microbial forensics and clinical applications to protein structure, dynamics and function, the following chapters written by leading experts serve as a forum for presenting some of the latest revolutionizing methods for mass spectrometry-based characterization of proteins and peptides. Staying faithful to the spirit of the Methods in Molecular Biology(TM) series, all chapters are written in the same format, whereby each chapter opens with a basic description of the method, followed by a description of the protocol and then practical step-by-step instructions to ensure experimental success.
Mass Spectrometry of Proteins and Peptides: Methods and Protocols, Second Edition is a valuable resource for novice and seasoned practitioners in the fields of biochemistry, molecular biology, clinical chemistry, immunology, genetic, microbiology and toxicology.
Mass Spectrometry of Proteins and Peptides: Methods and Protocols, Second Edition is a valuable resource for novice and seasoned practitioners in the fields of biochemistry, molecular biology, clinical chemistry, immunology, genetic, microbiology and toxicology.
Klappentext zu „Mass Spectrometry of Proteins and Peptides “
When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach - referred to as shotgun proteomics - is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.
When the last edition of this book was published in 2000, the field of proteomics was in its infancy. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. As a result of these rapid developments that have led to expanded areas of research and innovative applications, an update to Mass Spectrometry of Proteins and Peptides is not only worthwhile, but arguably mandatory. Spanning fields from microbial forensics and clinical applications to protein structure, dynamics and function, the following chapters written by leading experts serve as a forum for presenting some of the latest revolutionizing methods for mass spectrometry-based characterization of proteins and peptides. Staying faithful to the spirit of the Methods in Molecular Biology(TM) series, all chapters are written in the same format, whereby each chapter opens with a basic description of the method, followed by a description of the protocol and then practical step-by-step instructions to ensure experimental success.
Mass Spectrometry of Proteins and Peptides: Methods and Protocols, Second Edition is a valuable resource for novice and seasoned practitioners in the fields of biochemistry, molecular biology, clinical chemistry, immunology, genetic, microbiology and toxicology.
Mass Spectrometry of Proteins and Peptides: Methods and Protocols, Second Edition is a valuable resource for novice and seasoned practitioners in the fields of biochemistry, molecular biology, clinical chemistry, immunology, genetic, microbiology and toxicology.
Inhaltsverzeichnis zu „Mass Spectrometry of Proteins and Peptides “
- Preface- Contributors
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- Bobbie-Jo M. Webb-Robertson
- Generation and Analysis of Multidimensional Protein Identification Technology Datasets
- Selene K. Swanson, Laurence Florens and Michael P. Washburn
- Quantitative Peptide and Protein Profiling by Mass Spectrometry
- Alexander Schmidt, Birgit Bisle and Thomas Kislinger
- Label Free Relative Quantitation of Prokaryotic Proteomes Using the Accurate Mass and Time Tag Approach
- Kim K. Hixson
- Classical Proteomics: Two-dimensional Electrophoresis/MALDI Mass Spectrometry
- Ursula Zimny-Arndt, Monika Schmid, Renate Ackermann and Peter R. Jungblut
- The Use of Difference In-Gel Electrophoresis for Quantitation of Protein Expression
- Rajat Sapra
- Liquid-Chromatography-Mass Spectrometry of Thylakoid Membrane Proteins
- Christian G. Huber, Anna-Maria Timperio, Hansjörg Toll and Lello Zolla
- High Accuracy Mass Spectrometry in Large-Scale Analysis of Protein Phosphorylation
- Jesper V. Olsen and Boris Macek
- Manual Validation of Peptide Sequence and Sites of Tyrosine Phosphorylation from MS/MS Spectra
- Amy Nichols and Forest White
- Assigning Glycosylation Sites and Microheterogeneities in Glycoproteins by Liquid Chromatography/Tandem Mass Spectrometry
- Yehia Mechref, Milan Madera and Milos V. Novotny
- Structure Analysis of N-glycoproteins
- Stefanie Henning, Jasna Peter-Katalinic and Gottfried Pohlentz
- Capillary Zone Electrophoresis-mass Spectrometry for the Characterization of Isoforms of Intact Glycoproteins
- Christian Neusüss and Matthias Pelzing
- Top-down Proteomics on a High-field Fourier Transform Ion Cyclotron Resonance Mass spectrometer
- Séverine A. Ouvry-Patat, Matthew P. Torres, Craig Gelfand, Hung-Hiang Quek, Michael Easterling, J. Paul Speir and Christoph H. Borchers
- Capillary Isoelectric Focusing/Reversed Phase Liquid Chromatography/Mass Spectrometry
- Cheng S. Lee and Brian M. Balgley
- Integrating Accelerated Tryptic Digestion into Proteomics Workflows
- Gordon W. Slysz and David C. Schriemer
- Hydrogen/Deuterium Exchange Mass Spectrometry
- Xuguang Yan and Claudia S. Maier
- Mass Spectrometry Detection and Characterization of Noncovalent Protein Complexes
- Sheng Yin and Joseph A. Loo
- Chemical Cross-linking for Protein-protein Interaction Studies
- Xiaoting Tang and James E. Bruce
- Tissue Analysis with High Resolution Imaging Mass Spectrometry
- A.F. Maarten Altelaar and Ron M.A. Heeren
- Proteomic Global Profiling for Cancer Biomarker Discovery
- Samir Hanash
- Analysis of Protein Glycosylation and Phosphorylation Using Liquid Phase Separation, Protein Microarray Technology and Mass Spectrometry
- Jia Zhao, Tasneem H. Patwa, Manoj Pal, Weilian Qiu and David M. Lubman
- Transthyretin Mass Determination for Detection of Transthyretin Familial Amyloid
- John F. O'Brien and H. Robert Bergen, III
- Characterization of Microorganisms by MALDI Mass Spectrometry
- Catherine E. Petersen, Nancy B. Valentine and Karen L. Wahl
- Mass Spectrometric Characterization Of Neuropeptides
- Stephanie S. DeKeyser, James A. Dowell and Lingjun Li
- Peptide and Protein Ion/Ion Reactions in Electrodynamic Ion Traps: Tools and Methods
- Scott A. McLuckey
- Electron Capture Dissociation LC/MS/MS for Bottom-Up Proteomics
- Roman A. Zubarev
- Two-Dimensional Ion Mobility Analyses of Proteins and Peptides
- Alexandre A. Shvartsburg, Keqi Tang and Richard D. Smith
- Proteomics for Validation of Automated Gene Model Predictions
- Kemin Zhou, Ellen A. Panisko, Jon K. Magnuson, Scott E. Baker and Igor V. Grigoriev
- Support Vector Machines for Improved Peptide Identification from Tandem Mass Spectrometry Database Search
- Bobbie-Jo M. Webb-Robertson
... weniger
Bibliographische Angaben
- 2008, 2nd ed., XIV, 470 Seiten, Masse: 19,8 x 26,6 cm, Gebunden, Englisch
- Herausgegeben: Mary S. Lipton, Ljiljana Pasa-Tolic
- Verlag: Springer, Berlin
- ISBN-10: 1934115487
- ISBN-13: 9781934115480
- Erscheinungsdatum: 18.12.2008
Sprache:
Englisch
Rezension zu „Mass Spectrometry of Proteins and Peptides “
From the reviews of the second edition:
Pressezitat
From the reviews of the second edition: "This book ... covers a wide range of applications of modern mass spectrometry in peptide and protein analysis. ... Very useful is the presence of a final 'Notes' section within each chapter. These notes and laboratory tips help both beginners and experienced researchers to make their first steps into new fields ... . The well balanced mixture ... makes it a helpful resource for scientists working in analytical chemistry, biochemistry, biotechnology, and bioinformatics, and a valuable resource for everyone working in biologically oriented mass spectrometry." (Andreas Tholey, Analytical and Bioanalytical Chemistry, September, 2009)
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