Lung Innate Immunity and Inflammation

500 Million Alveoli from 30,000 Feet: A Brief Primer on Lung Anatomy.- Overview of Innate Lung Immunity and Inflammation.- Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes.- Isolation and Characterization of Mouse Neutrophils.- Isolation and Characterization of Mouse Fibroblasts.- Isolation of Rat and Mouse Alveolar Type II Epithelial Cells.-Isolation and Characterization of Human Alveolar type II Cells.- Air-liquid Interface Culture of Human and Mouse Airway Epithelial Cells.- Isolation and Characterization of Human Lung Myeloid Cells.-Measurement of Protein Permeability and Fluid Transport of Human Alveolar Epithelial Type II Cells under Pathological Conditions.- Measuring Innate Immune Function in Mouse Mononuclear Phagocytes.- Measuring Neutrophil Bactericidal Activity.- Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches.- Modulation of Lung Epithelial Cell Function Using Conditional and Inducible Transgenic Approaches.- Computational Analysis of RNA-seq Data from Airway Epithelial Cells for Studying Lung Disease.- Application of Proteomics in Lung Research.- Application of Metabolomics in Lung Research.- Functional Genomics in Murine Macrophages.- Assessment of Ozone-induced Lung Injury in Mice.- Lung Histological Methods.- Intravital Microscopy in the Mouse Lung.- Mouse Models of Acute Lung Injury and ARDS.- Mouse Models of Asthma.- Animal Models of Pulmonary Fibrosis.- Mouse Models of COPD.- Mouse Models of COPD.- Delivery of Therapeutics to the Lung.