Histone Variants

Preface...

Table of Contents...

Contributing Authors...

Part I Biochemistry

1. Methods for Preparing Nucleosomes Containing Histone Variants

Tomoya Kujirai, Yasuhiro Arimura, Risa Fujita, Naoki Horikoshi, Shinichi Machida, and Hitoshi Kurumizaka

2. Characterization of Post-Translational Modifications on Histone Variants

Francisco Saavedra, Sebastián Marty-Lombardi, and Alejandra Loyola

3. Purification of Histone Variant-Interacting Chaperone Complexes

Hideaki Tagami

4. Detection of Histone Modification Dynamics during the Cell Cycle by MS-Based Proteomics

Moritz Carl Völker-Albert, Andreas Schmidt, Teresa Barth, Ignasi Forne, and Axel Imhof

Part II Epigenomics

5. Histone Native Chromatin Immunprecipitation

Alicia Alonso, Emily Bernstein, and Dan Hasson

6. How to Tackle Challenging ChIP-seq, with Long-Range Cross-Linking, using ATRX as an Example

Julia Truch, Jelena Telenius, Douglas R. Higgs, and Richard J. Gibbons

7. Time-ChIP : A Method to Determine Long-Term Locus-Specific Nucleosome Inheritance

Wojciech Siwek, Mariluz Gómez-Rodríguez, Daniel Sobral, Ivan R. Corrêa, and Lars E.T. Jansen

8. MINCE-seq : Mapping In Vivo Nascent Chromatin with EdU and Sequencing

Srinivas Ramachandran and Steven Henikoff

9. RChIP-seq : Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes

Tatiana A. Soboleva and David J. Tremethick10. Bioinformatics Analysis of Nucleosome and Histone Variant Positioning

Jakub Mieczkowski and Michael Y. Tolstorukov

Part III Microscopy and Cell Biology

11. Imaging newly Synthesized and Old Histone Variant Dynamics Dependent on Chaperones Using the SNAP-Tag System

Júlia Torné, Guillermo Orsi, Dominique Ray-Gallet, and Geneviève Almouzni

12. Real Time de novo Deposition of Centromeric