Glyco-Engineering
Methods and Protocols
(Sprache: Englisch)
Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and...
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Klappentext zu „Glyco-Engineering “
Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.
Inhaltsverzeichnis zu „Glyco-Engineering “
Part I: Glyco-Engineering in Bacteria1. Current Approaches to Engineering N-Linked Protein Glycosylation in Bacteria
Andreas Naegeli and Markus Aebi
2. Inverse Metabolic Engineering for Enhanced Glycoprotein Production in Escherichia coli
Stephen R.P. Jaffé, Benjamin Strutton, Jagroop Pandhal, and Phillip C. Wright
3. GlycoSNAP: A High-Throughput Screening Methodology for Engineering Designer Glycosylation Enzymes
Anne A. Ollis, Yi Chai, and Matthew P. DeLisa
4. Production of Glycoproteins with Asparagine-Linked N-Acetylglucosamine in Escherichia coli
Flavio Schwarz and Markus Aebi
5. Glyco-Engineering O-Antigene-Based Vaccines and Diagnostics in E. coli
Nicolas F. Vozza and Mario F. Feldman
Part II: Glyco-Engineering in Yeast
6. Progress in Yeast Glycosylation Engineering
Stephen R. Hamilton and Dongxing Zha
7. Protein Production with a Pichia pastoris OCH1 Knock-Out Strain in Fed-Batch Mode
Christoph Gmeiner and Oliver Spadiut
8. Engineering the Pichia pastoris N-Glycosylation Pathway Using the GlycoSwitch Technology
Bram Laukens, Charlot De Wachter, and Nico Callewaert
9. Development of a Valuable Yeast Strain Using a Novel Mutagenesis Technique for the Effective Production of Therapeutic Glycoproteins
Hiroko Abe and Ken-ichi Nakayama
Part III: Glyco-Engineering in Insects
10. An Overview and History of Glyco-Engineering in Insect Expression Systems
Christoph Geisler, Hideaki Mabashi-Asazuma, and Donald L. Jarvis
11. SweetBac: Applying MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylation
Dieter Palmberger and Dubravko Rendic
12. Engineering
... mehr
Sialic Acid Synthesis Ability in Insect Cells
Karthik Viswanathan, Someet Narang, and Michael J. Betenbaugh
13. Engineering N-Glycosylation Pathway in Insect Cells: Suppression of beta-N-Acetylglucosaminidase and Expression of beta-1,4-Galactosyltransferase
Yeon Kyu Kim and Hyung Joon Cha
Part IV: Glyco-Engineering in Plants
14. N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements
Herta Steinkellner and Alexandra Castilho
15. Gene Targeting for Precision Glyco-Engineering: Production of Biopharmaceuticals Devoid of Plant-Typical Glycosylation in Moss Bioreactors
Eva L. Decker, Gertrud Wiedemann, and Ralf Reski
16. Im"plant"ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation
Hiroyuki Kajiura and Kazuhito Fujiyama
17. Transient Glyco-Engineering of N. benthamiana Aiming at the Synthesis of Multi-Antennary Sialylated Proteins
Andreas Loos and Alexandra Castilho
18. Subcellular Targeting of Proteins Involved in Modification of plant N- and O-Glycosylation
Martina Dicker, Jennifer Schoberer, Ulrike Vavra, and Richard Strasser
19. Assembly of Multigene Constructs Using Golden Gate Cloning
Sylvestre Marillonnet and Stefan Werner
Part V: Glyco-Engineering in Mammalians
20. Strategies for Engineering Protein N-Glycosylation Pathway in Mammalian Cells
Qiong Wang, Mark Stuczynski, Yuan Gao, and Michael Betenbaugh
21. Glycan Remodelling with Processing Inhibitors and Lectin-Resistant Eukaryotic Cells
Veronica T. Chang, Robert A. Spooner, Max Crispin, and Simon J. Davis
22. Production of Highly Sialylated Recombinant Glycoproteins Using Ricinus communis Agglutinin-I-Resistant CHO Glycosylation Mutants
John S.Y. Goh, Kah Fai Chan, and Zhiwei Song
23. Metabolic Glyco-Engineering in Eukaryotic Cells and Selected Applications
Friedrich Piller, Aline Mongis, and Véronique Piller
24. Evaluation of Quenching and Extraction Methods for Nucleotide/Nucleotide Sugar Analysis
Katrin Braasch, Carina Villacrés Barragán, and Michael Butler
Part VI: In Vitro Glyco-Engineering of Recombinant Proteins
25. Chemoenzymatic Glyco-Engineering of Monoclonal Antibodies
John P. Giddens and Lai-Xi Wang
26. Chemical Polysialylation of Recombinant Human Proteins
Ivan V. Smirnov, Ivan I. Vorobiev, Alexey A. Belogurov, Dmitry D. Genkin, Sergey M. Deyev, and Alexander G. Gabibov
Part VII: Glycan Analysis
27. Site Specific Glycosylation Profiling Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS)
Clemens Gruber and Friedrich Altmann
28. Mass Spectrometric Analysis of Oligo- and Polysialic Acids
Christina E. Galuska, Kai Maass, and Sebastian P. Galuska
29. Isomer-Specific Analysis of Released N-Glycans by LC-ESI MS/MS with Porous Graphitized Carbon
Daniel Kolarich, Markus Windwarder, Kathirvel Alagesan, and Friedrich Altmann
Karthik Viswanathan, Someet Narang, and Michael J. Betenbaugh
13. Engineering N-Glycosylation Pathway in Insect Cells: Suppression of beta-N-Acetylglucosaminidase and Expression of beta-1,4-Galactosyltransferase
Yeon Kyu Kim and Hyung Joon Cha
Part IV: Glyco-Engineering in Plants
14. N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements
Herta Steinkellner and Alexandra Castilho
15. Gene Targeting for Precision Glyco-Engineering: Production of Biopharmaceuticals Devoid of Plant-Typical Glycosylation in Moss Bioreactors
Eva L. Decker, Gertrud Wiedemann, and Ralf Reski
16. Im"plant"ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation
Hiroyuki Kajiura and Kazuhito Fujiyama
17. Transient Glyco-Engineering of N. benthamiana Aiming at the Synthesis of Multi-Antennary Sialylated Proteins
Andreas Loos and Alexandra Castilho
18. Subcellular Targeting of Proteins Involved in Modification of plant N- and O-Glycosylation
Martina Dicker, Jennifer Schoberer, Ulrike Vavra, and Richard Strasser
19. Assembly of Multigene Constructs Using Golden Gate Cloning
Sylvestre Marillonnet and Stefan Werner
Part V: Glyco-Engineering in Mammalians
20. Strategies for Engineering Protein N-Glycosylation Pathway in Mammalian Cells
Qiong Wang, Mark Stuczynski, Yuan Gao, and Michael Betenbaugh
21. Glycan Remodelling with Processing Inhibitors and Lectin-Resistant Eukaryotic Cells
Veronica T. Chang, Robert A. Spooner, Max Crispin, and Simon J. Davis
22. Production of Highly Sialylated Recombinant Glycoproteins Using Ricinus communis Agglutinin-I-Resistant CHO Glycosylation Mutants
John S.Y. Goh, Kah Fai Chan, and Zhiwei Song
23. Metabolic Glyco-Engineering in Eukaryotic Cells and Selected Applications
Friedrich Piller, Aline Mongis, and Véronique Piller
24. Evaluation of Quenching and Extraction Methods for Nucleotide/Nucleotide Sugar Analysis
Katrin Braasch, Carina Villacrés Barragán, and Michael Butler
Part VI: In Vitro Glyco-Engineering of Recombinant Proteins
25. Chemoenzymatic Glyco-Engineering of Monoclonal Antibodies
John P. Giddens and Lai-Xi Wang
26. Chemical Polysialylation of Recombinant Human Proteins
Ivan V. Smirnov, Ivan I. Vorobiev, Alexey A. Belogurov, Dmitry D. Genkin, Sergey M. Deyev, and Alexander G. Gabibov
Part VII: Glycan Analysis
27. Site Specific Glycosylation Profiling Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS)
Clemens Gruber and Friedrich Altmann
28. Mass Spectrometric Analysis of Oligo- and Polysialic Acids
Christina E. Galuska, Kai Maass, and Sebastian P. Galuska
29. Isomer-Specific Analysis of Released N-Glycans by LC-ESI MS/MS with Porous Graphitized Carbon
Daniel Kolarich, Markus Windwarder, Kathirvel Alagesan, and Friedrich Altmann
... weniger
Bibliographische Angaben
- 2015, 2015, XVI, 439 Seiten, 52 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Alexandra Castilho
- Verlag: Springer, Berlin
- ISBN-10: 1493927590
- ISBN-13: 9781493927593
- Erscheinungsdatum: 17.06.2015
Sprache:
Englisch
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