Genomics Protocols

Contents Preface Contributors 1. Whole Genome Amplification with Phi29 DNA Polymerase to enable Genetic or Genomic Analysis of Samples of Low DNA Yield Kaisa Silander.................................................................................... 2. Scanning for DNA variants by denaturant capillary electrophoresis Per O. Ekstrøm................................................................................... 3. Identification of SNPs, or Mutations in Sequence Chromatograms Nicole Draper..................................................................................... 4. Beadarray based Genotyping Helen Butler and Jiannis Ragoussis...................................................... 5. Microsatellite-Based Candidate Gene Linkage Analysis Studies Cathryn Mellersh................................................................................. 6. Full Complexity Genomic Hybridization on 60mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH) Alexei Protopopov, Bin Feng, and Lynda Chin....................................... 7. Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin Fixed, Paraffin-Embedded Tissue by Multiplex Ligation- Dependent Probe Amplification Minoru Takata.................................................................................... 8. Application of Microarrays for DNA Methylation Profiling Axel Schumacher, Andreas Weinhäusl, and Arturas Petronis.................. 9. Genome-Wide Identification of Protein Binding Locations using Chromatin Immunoprecipitation coupled with Microarray Byung-Kwan Cho, Eric M. Knight, and Bernhard Ø. Palsson.................... 10. Transcriptional Profiling of Small Samples in the CentralNervous System Stephen D. Ginsberg........................................................................... 11. Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin- Embedded Tissues using randomly assembled Bead Arrays Marina Bibikova, Joanne M.

From the reviews of the second edition:"The main purpose is to present current protocols in genomic research, and the book provides a comprehensive update on this area of research ... . The book is written primarily for scientists in the field of genomic research. ... presents a good balance between the different aspects involved in the study of functional genomics. It also provides a number of updated protocols for practicing investigators in the field of genomic research. ... a valuable companion to all researchers in the field of genomic research." (Ruben Mestril, Doody's Review Service, January, 2009)