Detection of Blotted Proteins
Methods and Protocols
(Sprache: Englisch)
This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional...
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This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting.
Inhaltsverzeichnis zu „Detection of Blotted Proteins “
1. Western Blotting: Origin and Ascent of the SpeciesW. Neal Burnette
2. Methods to Concentrate Proteins for Protein Isolation, Proteomic and Peptidomic Evaluation
J.P. Dean Goldring
3. Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose
J.P. Dean Goldring
4. Detection of Blotted Proteins: Not all Blockers are Created Equal
Vishal Kothari and Suresh T. Mathews
5. Protein Stains to Detect Antigen on Membranes
Anil D'souza and Hal Scofield
6. Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting
F. Javier Alba and Salvador Bartolomé
7. Rapid, Antibody-free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation
Neil W. Charter, Joe Horecka, Chin-Yee Loh, Albert Doan, Tom Wehrman, and Keith R. Olson
8. Use of Non-Radioactive Detection Method for North- and South-Western Blot
Claudia Franke, Daniel Gräfe, Holger Bartsch, Michael Bachmann
9. Immunoblotting using Radiolabeled Reagents for Detection
Holger Bartsch, Claudia Franke, Michael Bachmann
10. Immunoblotting of Antigens: Whole, Strip and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique
Yaser Dorri
11. Detection of Protein Carbonyls by Means of Biotin Hydrazide -Streptavidin Affinity Methods
Kenneth Hensley
12. Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel
Surbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend
13. Quantitative Analysis of Signal Transduction with In-Cell Western Immunofluorescence Assays
Vince Boveia and Amy Schutz-Geschwender
14. Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots
Fangmao Ye, Polina B Smith, and Daniel T. Chiu
15. Co-detection of Target and Total Protein by CyDye Labeling and Fluorescent ECL-plex Immunoblotting in a Standard Proteomics Workflow
Caitriona Scaife,
... mehr
Ciara A. McManus, Pamela M. Donoghue, and Michael J. Dunn
16. Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot blots and Immunohistochemistry
Thomas Millar, Ronald Knighton and Jo-Anne Chuck
17. Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane
Biji T. Kurien and Michael Bachmann
18. Sequential use of Immunoblots for Characterization of Autoantibody Specificities
Holger Bartsch and Michael Bachmann
19. Nanogold Immunodetection Detection Systems for the Identification of Autoantigens by Western Blotting
Jacen S. Moore and R. Hal Scofield
20. Application of Intermittent Microwave Irradiation to Western Blot Analysis
Yu-Ting Liu and Shinya Toyokuni
21. Visualization of Unstained Protein Bands on PVDF
Jun Park, Masaharu Mabuchi, and Ajay Sharma
22. Multiplexed Fluorescent Immunodetection Using Low Auto-Fluorescence Immobilon®-FL Membrane
Jun Park, Masaharu Mabuchi, and Ajay Sharma
23. Cold Microwave-Enabled Protein Detection and Quantification
Niels Grützner, Romy M. Heilmann, Aaron G. Smith, Carol B. Johnson, Stanislav Vitha, Jörg M. Steiner, and Andreas K. Holzenburg
24. TLC-Blot (Far-Eastern Blot) and Its Application to Functional Lipidomics
Takao Taki
25. Analysis of Electroblotted Proteins by Mass Spectrometry
Jose L. Luque-Garcia and Thomas A. Neubert
26. On Membrane Renaturation of Recombinant Ro60 Autoantigen by Calcium Ions
Biji T. Kurien and Michael P. Bachmann
27. Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore
Lee Broderick Bockus and R. Hal Scofield
28. Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane
Biji T. Kurien and R. Hal Scofield
29. Detection of Blotted Proteins on Nitrocellulose/PVDF Membranes by Alta
Jayanta K. Pal, Shilpa J. Rao, and Dhanashri J. Godbole
30. Non-stripping "Rainbow" and Multiple Antigen Detection (MAD) Western Blotting
Stan (Stanislaw) Krajewski, Michelle M. Tsukamoto, Xianshu Huang, and Sebastian B. Krajewski
31. Supported Molecular Matrix Electrophoresis
Yu-ki Matsuno and Akihiko Kameyama
32. Parafilm-M®, an Available Cost Effective Alternative for Immuno-blot Pouches
Syed MS Quadri
33. Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membranes
Akihiko Kameyama, Weijie Dong, and Yu-ki Matsuno
34. Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in Saliva
Suresh T. Mathews, Emily Graff, Robert L. Judd, and Vishal Kothari, M.Pharm
35. A Novel Methodology for Stripping and Reprobing of Western Blots Originally Developed with Colorimetric Substrate TMB
Parmita Kar, Saurabh Kumar Agnihotri, Archana Sharma, Rekha Sachan , and Madan Lal Bhatt, Monika Sachdev
36. Other Notable Methods of Membrane Protein Detection: A Brief Review
Biji T. Kurien and R. Hal Scofield
37. Nitrocellulose Membrane: The New Canvas
Jasmin R. Kurien and Bianca A Kurien
38. Invisible Ink Marking in ECL Membrane Assays
Biji T. Kurien
16. Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot blots and Immunohistochemistry
Thomas Millar, Ronald Knighton and Jo-Anne Chuck
17. Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane
Biji T. Kurien and Michael Bachmann
18. Sequential use of Immunoblots for Characterization of Autoantibody Specificities
Holger Bartsch and Michael Bachmann
19. Nanogold Immunodetection Detection Systems for the Identification of Autoantigens by Western Blotting
Jacen S. Moore and R. Hal Scofield
20. Application of Intermittent Microwave Irradiation to Western Blot Analysis
Yu-Ting Liu and Shinya Toyokuni
21. Visualization of Unstained Protein Bands on PVDF
Jun Park, Masaharu Mabuchi, and Ajay Sharma
22. Multiplexed Fluorescent Immunodetection Using Low Auto-Fluorescence Immobilon®-FL Membrane
Jun Park, Masaharu Mabuchi, and Ajay Sharma
23. Cold Microwave-Enabled Protein Detection and Quantification
Niels Grützner, Romy M. Heilmann, Aaron G. Smith, Carol B. Johnson, Stanislav Vitha, Jörg M. Steiner, and Andreas K. Holzenburg
24. TLC-Blot (Far-Eastern Blot) and Its Application to Functional Lipidomics
Takao Taki
25. Analysis of Electroblotted Proteins by Mass Spectrometry
Jose L. Luque-Garcia and Thomas A. Neubert
26. On Membrane Renaturation of Recombinant Ro60 Autoantigen by Calcium Ions
Biji T. Kurien and Michael P. Bachmann
27. Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore
Lee Broderick Bockus and R. Hal Scofield
28. Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane
Biji T. Kurien and R. Hal Scofield
29. Detection of Blotted Proteins on Nitrocellulose/PVDF Membranes by Alta
Jayanta K. Pal, Shilpa J. Rao, and Dhanashri J. Godbole
30. Non-stripping "Rainbow" and Multiple Antigen Detection (MAD) Western Blotting
Stan (Stanislaw) Krajewski, Michelle M. Tsukamoto, Xianshu Huang, and Sebastian B. Krajewski
31. Supported Molecular Matrix Electrophoresis
Yu-ki Matsuno and Akihiko Kameyama
32. Parafilm-M®, an Available Cost Effective Alternative for Immuno-blot Pouches
Syed MS Quadri
33. Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membranes
Akihiko Kameyama, Weijie Dong, and Yu-ki Matsuno
34. Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in Saliva
Suresh T. Mathews, Emily Graff, Robert L. Judd, and Vishal Kothari, M.Pharm
35. A Novel Methodology for Stripping and Reprobing of Western Blots Originally Developed with Colorimetric Substrate TMB
Parmita Kar, Saurabh Kumar Agnihotri, Archana Sharma, Rekha Sachan , and Madan Lal Bhatt, Monika Sachdev
36. Other Notable Methods of Membrane Protein Detection: A Brief Review
Biji T. Kurien and R. Hal Scofield
37. Nitrocellulose Membrane: The New Canvas
Jasmin R. Kurien and Bianca A Kurien
38. Invisible Ink Marking in ECL Membrane Assays
Biji T. Kurien
... weniger
Bibliographische Angaben
- 2015, 2015, XIV, 386 Seiten, 48 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Biji T. Kurien, R. Hal Scofield
- Verlag: Springer, Berlin
- ISBN-10: 1493927175
- ISBN-13: 9781493927173
- Erscheinungsdatum: 04.07.2015
Sprache:
Englisch
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