Chromatographic Methods
(Sprache: Englisch)
Since the inception of chromatography in 1903, the principal landmarks in its progress have been the virtual rediscovery of the technique in the 1930s, invention of synthetic resins in 1935, introduction of paper chromatography in the 1940s followed by that...
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Klappentext zu „Chromatographic Methods “
Since the inception of chromatography in 1903, the principal landmarks in its progress have been the virtual rediscovery of the technique in the 1930s, invention of synthetic resins in 1935, introduction of paper chromatography in the 1940s followed by that of thin layer, gas-solid and gas-liquid chroma tography in the early 1950s. Whilst the theoretical aspects of HPLC were developed in the 1960s, it was the late 1970s before commercial instruments appeared. Developments through the 1980s in microelectronics and micro processor technology afforded enhanced control, data acquisition and processing capabilities, and improved technologies for the manufacture of instrumentation. Developments in chromatographic media and packings and rapid scanning spectroscopic instruments have enabled combination techniques such as GC-MS, GC-IR, HPLC-MS and HPLC-IR to reach maturity and become standard routine techniques for the analyst. Further considerable research activity in the 1980s and early 1990s has led to the development of supercritical fluid chromatography (SFC), and high perfor mance capillary electrophoresis is a technique that has proved invaluable in the genome project and the separation and typing of DNA fragments. Applications in environmental, health and safety, foods analysis and medical studies have contributed significantly to the advancement of these tech niques. All of the instrumental chromatographic techniques are now used routinely by academic and industrial analysts. An understanding and experi ence of such techniques is fundamental to the training of today's science undergraduates studying a range of disciplines reflecting the application areas mentioned above.
Inhaltsverzeichnis zu „Chromatographic Methods “
1 Introduction.- 1.1 Introduction to chromatography.- 1.2 History of chromatography.- 1.3 Classification of chromatographic methods.- References.- 2 Theoretical considerations.- 2.1 Introduction.- 2.2 Factors influencing retention.- 2.3 Retention and equilibrium in chromatography.- 2.4 Separating efficiency of a column.- 2.5 Band broadening processes.- 2.6 Resolution.- 2.7 Quantification in chromatography.- References.- 3 Planar chromatography.- 3.1 Thin layer chromatography.- 3.2 Paper chromatography.- 3.3 Electrophoresis.- 3.4 Capillary electrophoresis.- References.- 4 Liquid phase chromatography on open columns.- 4.1 Introduction.- 4.2 Practical aspects and considerations.- 4.3 Modes of chromatography.- 4.4 Adsorption chromatography.- 4.5 Partition column chromatography.- 4.6 Ion exchange chromatography.- 4.7 Inorganic ion exchangers.- 4.8 Gel ion exchangers.- 4.9 Gel chromatography.- 4.10 Affinity chromatography.- 4.11 Covalent chromatography.- 4.12 Chromatofocusing.- 4.13 Flash chromatography.- References.- 5 Gas chromatography.- 5.1 Introduction.- 5.2 Principles of gas chromatography.- 5.3 Resolution.- 5.4 Columns and stationary phase.- 5.5 Choice of stationary phase.- 5.6 Capillary column gas chromatography.- 5.7 Gas-solid chromatography.- 5.8 Gas chromatography instrumentation.- 5.9 Sampling techniques.- 5.10 Detectors for gas chromatography.- 5.11 Supercritical gas chromatography.- 5.12 Applications of gas chromatography.- References.- 6 High performance liquid chromatography.- 6.1 Introduction.- 6.2 Modes of chromatography.- 6.3 Overview of high performance liquid chromatography instrumentation.- 6.4 Theory.- 6.5 Detailed discussion of high performance liquid chromatography instrumentation.- 6.6 Column packings and stationary phases for liquid chromatography.-6.7 Adsorption chromatography.- 6.8 Liquid-liquid partition chromatography.- 6.9 Chemically bonded stationary phases for high performance liquid chromatography.- 6.10 Chiral chromatography.- 6.11 Ion
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exchange chromatography.- 6.12 Ion pairing.- 6.13 Size exclusion chromatography.- 6.14 Liquid chromatography method development.- 6.15 Quantitative analysis.- 6.16 Preparative liquid chromatography.- 6.17 Microcolumns in liquid chromatography.- 6.18 Applications.- References.- 7 Chromatography and spectroscopic techniques.- 7.1 Introduction.- 7.2 Chromatographic requirements.- 7.3 Chromatographic and mass spectrometry techniques.- 7.4 Gas chromatography interfacing techniques.- 7.5 High performance liquid chromatography interfacing.- 7.6 Chromatography and mass spectrometry data systems.- 7.7 Infrared spectrophotometry.- 7.8 Ultraviolet-visible spectrophotometry.- 7.9 Atomic spectroscopy.- References.- 8 Processing chromatographic data.- 8.1 Introduction.- 8.2 The chromatogram.- 8.3 Data collection and processing.- 8.4 Chromatography software.- 8.5 Method development and optimisation.- 8.6 Laboratory information management systems.- References.- 9 Model or practical experiments in chromatographic techniques.- 9.1 Introduction.- 9.2 List of experiments.- 9.3 Section A. Paper chromatography.- 9.4 Section B. Electrophoresis and related techniques.- 9.5 Section C. Thin layer chromatography.- 9.6 Section D. Column chromatography.- 9.7 Section E. Gas chromatography.- 9.8 Section F. High performance liquid chromatography.- 9.9 Section G. Capillary electrophoresis.- References.- 10 Illustrative problems in chromatography.- 10.1 Introduction.- 10.2 Plate theory and resolution.- 10.3 Van Deemter.- 10.4 Volatility and temperature in gas chromatography.- 10.5 Qualitativeanalysis.- 10.6 Quantitative analysis.- 10.7 Composite questions.- Appendix 1 Glossary of chromatographic terms.- Appendix 2 Table of chromatography symbols.
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Bibliographische Angaben
- Autoren: A. Braithwaite , J.F. Smith
- 1995, 5th ed., 580 Seiten, Masse: 15,5 x 23,5 cm, Kartoniert (TB), Englisch
- Herausgegeben: A. Braithwaite, F. J. Smith
- Verlag: Springer Netherlands
- ISBN-10: 0751401587
- ISBN-13: 9780751401585
- Erscheinungsdatum: 31.12.1995
Sprache:
Englisch
Rezension zu „Chromatographic Methods “
comprehensive and well presented...The chapters are informative and easy ot read...already used widely in teaching institutions and this use will undoubtedly continue in the future because it provides an ideal resource for this purpose. I commend it highly - Journal of Chromatography
Pressezitat
comprehensive and well presented...The chapters are informative and easy ot read...already used widely in teaching institutions and this use will undoubtedly continue in the future because it provides an ideal resource for this purpose. I commend it highly - Journal of Chromatography
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