Biological Low-Voltage Scanning Electron Microscopy
(Sprache: Englisch)
This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, to teach the reader how to obtain the best quality images.
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This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, to teach the reader how to obtain the best quality images.
Klappentext zu „Biological Low-Voltage Scanning Electron Microscopy “
Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatial resolution and minimized sample charging and damage. Images produced are less destroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM.Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapter s in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.
Inhaltsverzeichnis zu „Biological Low-Voltage Scanning Electron Microscopy “
Table of Contents1. Low Voltage Scanning Electron Microscopy In Biology--Historical Preface :The Early Development of the Scanning Electron Microscope to 1965...1Dennis McMullan2. LVSEM For Biology...27James B. Pawley, PhD3. The Aberration-Corrected SEM...101David Joy Science and Engineering Research Facility, University of Tennessee4. Noise and its effects on the Low Voltage SEM...121David C Joy5. High-Resolution Low Voltage Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols...146Heide Schatten, PhD6. Molecular labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM...173Ralph Albrecht and Daryl Meyer7. Low kV and video-rate, beam-tilt stereo for viewing live-time experiments in the SEM...209Alan Boyde8. CryoSEM of Chemically Fixed Animal Cells...228Stanley L. Erlandsen 9. High resolution and low voltage SEM of plant cells...249Guy Cox, Peter Vesk, Teresa Dibbayawan, Tobias I. Baskin and Maret Vesk10. High Resolution Cryo-Scanning Electron Microscopy of Biological Samples...268Paul Walther11. Developments in Instrumentation for Microanalysis in Low Voltage Scanning Electron Microscopy...305Dale E. Newbury
Bibliographische Angaben
- 2007, 2008, 317 Seiten, Masse: 16 x 24,1 cm, Gebunden, Englisch
- Herausgegeben:Schatten, Heide; Pawley, James
- Herausgegeben: Heide Schatten, James Pawley
- Verlag: Springer
- ISBN-10: 0387729704
- ISBN-13: 9780387729701
- Erscheinungsdatum: 18.12.2007
Sprache:
Englisch
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