Auditory and Vestibular Research

Helios® Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: Recent Updates.- Tol2-Mediated Delivery of miRNAs to the Chicken Otocyst using Plasmid Electroporation.- A Rapid, Cost-Effective Method of Recombinant Adeno-Associated Virus Preparation Enables Efficient Gene Transfer to the Developing Mouse Inner Ear.- Generation of Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannoma and Meningioma.- RNA Extraction from Xenopus Auditory and Vestibular Organs for Molecular Cloning and Expression Profiling with RNA-Seq and Microarrays .- Yeast Two-Hybrid Screening to Test for Protein-Protein Interactions in the Auditory System.- Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research.- Protein Quantification of the Developing Cochlea by Mass Spectrometry.- Ultrastructural Identification and Colocalization of Interacting Proteins in the Murine Cochlea by Post-Embedding Immunogold Transmission Electron Microscopy.- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins.- The Single-Molecule Approach to Membrane Protein Stoichiometry.- Visualization of Live Cochlear Stereocilia at a Nanoscale Resolution Using Hopping Probe Ion Conductance Microscopy.- Design and Construction of a Cost-Effective Spinning Disk System for Live Imaging of Inner Ear Tissue.- Neuroanatomical Tracing Techniques: History, State of the Art, and Future Developments.- Detection of Excitation and Inhibition in the Auditory System Using Fluorescence Immunohistochemistry and High Resolution Fluorescence Microscopy.- Rapid and Semi-Automated Extraction of Neuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks.-Organotypic Culture of the Mouse Cochlea from Embryonic Day 12 to the Neonate.- Quantifying Spiral Ganglion Neurite and Schwann Behavior on Micropatterned Polymer Substrates.- The Use of Human Wharton's Jelly Cells for Cochlear Tissue Engineering.- Nanotechnology