Atomic Force Microscopy in Biomedical Research
Methods and Protocols
(Sprache: Englisch)
With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic...
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With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use. Written in the Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.
Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.
Klappentext zu „Atomic Force Microscopy in Biomedical Research “
With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use. Written in the Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.
Inhaltsverzeichnis zu „Atomic Force Microscopy in Biomedical Research “
Part I: The Basics of Atomic Force Microscopy 1. How the Atomic Force Microscope Works - Bruno Torre, Davide Ricci, and Pier Carlo Braga
2. Measurement Methods in Atomic Force Microscopy - Bruno Torre, Claudio Canale, Davide Ricci, and Pier Carlo Braga
3. Recognizing and Avoiding Artifacts in Atomic Force Microscopy Imaging - Claudio Canale, Bruno Torre, Davide Ricci, and Pier Carlo Braga
Part II: Molecule Imaging
4. Imaging the Spatial Orientation of Subunits within Membrane Receptors by Atomic Force Microscopy - Stewart M Carnally, J. Michael Edwardson, and Nelson P. Barrera
5. High Resolution Imaging of Immunoglobulin G (IgG) Antibodies and Other Biomolecules Using Amplitude Modulation Atomic Force Microscopy in Air - Sergio Santos and Neil H. Thomson
6. Atomic Force Microscopy of Ex vivo Amyloid Fibrils - Claudio Canale, Annalisa Relini, and Alessandra Gliozzi
7. Studying Collagen Self-Assembly by Time-Lapse High-Resolution Atomic Force Microscopy - Clemens M. Franz and Daniel J. Muller
8. Atomic Force Microscopy Imaging of Human Metaphase Chromosomes in Liquid - Osamu Hoshi and Tatsuo Ushiki
9. Atomic Force Microscopy of Proteasome Assemblies - Maria Gaczynska and Pawel A. Osmulski
10. Atomic Force Microscopy of Isolated Mitochondria - Bradley E. Layton and M. Brent Boyd
11. Imaging Native Membrane Proteins Using the Atomic Force Microscope - Andreas Engel
Part III: Nanoscale Surface Analysis and Cell Imaging
12. Atomic Force Microscopy Investigation of Viruses - Alexander McPherson and Yurii G. Kuznetsov
13. Determination of the Kinetic On and -Off Rate of Single Virus-Cell Interactions - Christian Rankl, Linda Wildling, Isabel Neundlinger, Ferry Kienberger, Hermann Gruber, Dieter Blaas, and Peter Hinterdorfer
14. Atomic Force Microscopy as a Tool for the Study of the Ultrastructure of Trypanosomatid Parasites - Wanderley de Souza, Gustavo M. Rocha, Kildare Miranda, Paulo M. Bisch, and Gilberto
... mehr
Weissmuller
15. Normal and Pathological Erythrocytes Studied by Atomic Force Microscopy - Andreas Ebner, Hermann Schillers, and Peter Hinterdorfer
16. The Growth Cones of Living Neurons Probed by the Atomic Force Microscope - Davide Ricci, Massimo Grattarola, and Mariateresa Tedesco
17. Highlights on Ultrastructural Pathology of Human Sperm - Narahari V. Joshi, Ibis Cruz, and Jesus A. Osuna
18. High-speed Atomic Force Microscopy and Biomolecular Processes - Takayuki Uchihashi and Toshio Ando
Part IV: Non-Topographical Applications (Force-Spectroscopy)
19. Atomic Force Microscopy in Mechanobiology: Measuring Micro-Elastic Heterogeneity of Living Cells - Evren U. Azeloglu and Kevin D. Costa
20. Force-Clamp Measurements of Receptor-Ligand Interactions - Félix Rico, Calvin Chu, and Vincent T. Moy
21. Measuring Cell Adhesion Forces: Theory and Principles - Martin Benoit and Christine Selhuber-Unkel
22. Nanoscale Investigation on E. coli Adhesion to Modified Silicone Surfaces - Ting Cao, Haiying Tang, Xuemei Liang, Anfeng Wang, Gregory W. Auner, Steven O. Salley, and K.Y. Simon Ng
Part V: Investigating Drug Action
23. Imaging Bacterial Shape, Surface, and Appendages Before and After Treatment with Antibiotics - Pier Carlo Braga and Davide Ricci
24. Thymol-Induced Alterations in Candida albicans Imaged by Atomic Force Microscopy - Pier Carlo Braga and Davide Ricci
25. Atomic Force Microscope-Enabled Studies of Integrin-Extracellular Matrix Interactions in Vascular Smooth Muscle and Endothelial Cells - Zhe Sun and Gerald A. Meininger
26. Atomic Force Microscopy Studies on Circular DNA Structural Changes by Vincristine and Aspirin - Zhongdang Xiao, Lili Cao, Dan Zhu, and Zuhong Lu
Part VI: Atomic Force Microscopy as a Nanotool
27. Combined Atomic Force Microscopy and Fluorescence Microscopy - Miklós S.Z. Kellermayer
28. Chemical Modifications of Atomic Force Microscopy Tips - Régis Barattin and Normand Voyer
29. Atomic Force Microscopy as Nanorobot - Ning Xi, Carmen Kar Man Fung, Ruiguo Yang, King Wai Chiu Lai, Donna H. Wang, Kristina Seiffert-Sinha, Animesh A. Sinha, Guangyong Li, and Lianqing Liu
15. Normal and Pathological Erythrocytes Studied by Atomic Force Microscopy - Andreas Ebner, Hermann Schillers, and Peter Hinterdorfer
16. The Growth Cones of Living Neurons Probed by the Atomic Force Microscope - Davide Ricci, Massimo Grattarola, and Mariateresa Tedesco
17. Highlights on Ultrastructural Pathology of Human Sperm - Narahari V. Joshi, Ibis Cruz, and Jesus A. Osuna
18. High-speed Atomic Force Microscopy and Biomolecular Processes - Takayuki Uchihashi and Toshio Ando
Part IV: Non-Topographical Applications (Force-Spectroscopy)
19. Atomic Force Microscopy in Mechanobiology: Measuring Micro-Elastic Heterogeneity of Living Cells - Evren U. Azeloglu and Kevin D. Costa
20. Force-Clamp Measurements of Receptor-Ligand Interactions - Félix Rico, Calvin Chu, and Vincent T. Moy
21. Measuring Cell Adhesion Forces: Theory and Principles - Martin Benoit and Christine Selhuber-Unkel
22. Nanoscale Investigation on E. coli Adhesion to Modified Silicone Surfaces - Ting Cao, Haiying Tang, Xuemei Liang, Anfeng Wang, Gregory W. Auner, Steven O. Salley, and K.Y. Simon Ng
Part V: Investigating Drug Action
23. Imaging Bacterial Shape, Surface, and Appendages Before and After Treatment with Antibiotics - Pier Carlo Braga and Davide Ricci
24. Thymol-Induced Alterations in Candida albicans Imaged by Atomic Force Microscopy - Pier Carlo Braga and Davide Ricci
25. Atomic Force Microscope-Enabled Studies of Integrin-Extracellular Matrix Interactions in Vascular Smooth Muscle and Endothelial Cells - Zhe Sun and Gerald A. Meininger
26. Atomic Force Microscopy Studies on Circular DNA Structural Changes by Vincristine and Aspirin - Zhongdang Xiao, Lili Cao, Dan Zhu, and Zuhong Lu
Part VI: Atomic Force Microscopy as a Nanotool
27. Combined Atomic Force Microscopy and Fluorescence Microscopy - Miklós S.Z. Kellermayer
28. Chemical Modifications of Atomic Force Microscopy Tips - Régis Barattin and Normand Voyer
29. Atomic Force Microscopy as Nanorobot - Ning Xi, Carmen Kar Man Fung, Ruiguo Yang, King Wai Chiu Lai, Donna H. Wang, Kristina Seiffert-Sinha, Animesh A. Sinha, Guangyong Li, and Lianqing Liu
... weniger
Bibliographische Angaben
- 2011, XIV, 508 Seiten, 5 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Pier C. Braga, Davide Ricci
- Verlag: Springer, Berlin
- ISBN-10: 1617791040
- ISBN-13: 9781617791048
Sprache:
Englisch
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