Antimicrobial and antitumor substance(s) produced by Actinomycetes
Microbial biotechnology
(Sprache: Englisch)
The emergence of extensive antibiotics resistant bacteria increased the demands for find out new source of antimicrobial agent, Actinomycetes, especially Streptomycetes spp have recently grasped considerable attention worldwide due to production of many...
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The emergence of extensive antibiotics resistant bacteria increased the demands for find out new source of antimicrobial agent, Actinomycetes, especially Streptomycetes spp have recently grasped considerable attention worldwide due to production of many useful bioactive metabolites that exploited by the pharmaceutical industry. In present study, a number of 52 Actinomycetes were recovered, from which 20 showed antimicrobial activities to at least one tested organism. The most potent isolate AGM12-1, which was active against all tested organisms and with IC50 3.3 and 1.1mig/ml. against HCT 116 and HepG-2 respectively was characterized as Streptomyces vinaceusdrappus with presence of ARO-PKS II genes. Mannitol, ammonium sulfate, pH 7, 2% stating inoculums and incubation for 11 days at 30°c were the optimum conditions used to maximize the production and hence allowed purification of one active compound to homogeneity using RP-HPLC with m/z 488.05.The NMR structural elucidation showed that this compound was a derivative of Diketopiperazine.
Autoren-Porträt von Ahmed Gaber Merdash, Maged S. Ahmad - Rasha R. Ahmed, Ahmed O. El-Gendy - Hussein M. El-Kabbany
Gaber Merdash, AhmedMicrobiologist Ahmed Gaber Merdash was born on 1 Oct. 1985. He graduated in Microbiology dep., Alazher University, Egypt (2006). He got his M.Sc (2016) in microbiology science From Faculty of science, Beni Suef university, Egypt. He is Ph.D candidate in Medical Microbiology science at Faculty of science, Alazher University, Egypt.
Bibliographische Angaben
- Autoren: Ahmed Gaber Merdash , Maged S. Ahmad - Rasha R. Ahmed , Ahmed O. El-Gendy - Hussein M. El-Kabbany
- 2019, 144 Seiten, Masse: 22 cm, Kartoniert (TB), Englisch
- Verlag: LAP Lambert Academic Publishing
- ISBN-10: 6139928273
- ISBN-13: 9786139928279
Sprache:
Englisch
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