Amyloid Proteins
Methods and Protocols
(Sprache: Englisch)
This third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that were not addressed in the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro...
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This third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that were not addressed in the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro assays that focus on a variety of amyloids and how to study these peptides and proteins. Part Two describes cell culture models and assays, and Part Three explores methods on how to extract amyloid from tissue, its detection, and its characterization in vitro or in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Amyloid Proteins: Methods and Protocols, Third Edition is a valuable resource for both students and scientists who are new to the field, as well as experienced researchers who would like to progress their research with the latest available techniques.
Inhaltsverzeichnis zu „Amyloid Proteins “
Preface...Table of Contents...
Contributing Authors...
Part I: In Vitro Models and Assays
1. Preparation of Pure Populations of Amyloid-ss Protein Oligomers of Defined Size
Eric Y. Hayden, Joseph L. Conovaloff, Ashley Mason, Gal Bitan, and David B. Teplow
2. Preparation of Well-Defined and Stable ss-Barrel Pore-Forming Amyloid-ss42 Oligomers
Montserrat Serra-Batiste, Martí Ninot-Pedrosa, Eduard Puig, Sonia Ciudad, Margarida Gairí, and Natàlia Carulla
3. Unveiling Brain Ass Heterogeneity through Targeted Proteomic Analysis
Agueda Rostagno, Thomas A. Neubert, and Jorge Ghiso
4. Preparation of a-Synuclein Amyloid Assemblies for Toxicity Experiments
Serene W. Chen and Nunilo Cremades
5. Generation and Characterization of Stable a-Synuclein Oligomers
Leire Almandoz-Gil, Martin Ingelsson, and Joakim Bergström
6. In Vitro Analysis of a-Synuclein Amyloid Formation and Cross-Reactivity
Istvan Horvath, Sandra Rocha, and Pernilla Wittung-Stafshede
7. Preparation of Tau Oligomers after Protein Extraction from Bacteria and Brain Cortices
Elentina K. Argyrousi, Agnieszka Staniszewski, Russell E. Nicholls, and Ottavio Arancio
8. Purification and Characterization of Low-n Tau Oligomers
Senthilvelrajan Kaniyappan, Ram Reddy Chandupatla, and Eckhard Mandelkow
9. Preparation and Characterization of Tau Oligomer Strains
Urmi Sengupta, Mariana Carretero-Murillo, and Rakez Kayed
10. Purification and Fibrillation of Recombinant Human Amyloid-ss, Prion Protein and Tau under Native Conditions
Alexander Sandberg and Sofie Nyström
11. Preparation of Amyloidogenic Aggregates from EF-Hand ss -parvalbumin and S100 Proteins
Javier Martínez, Joana S. Cristóvão, Rosa Sánchez, Maria Gasset , and Cláudio M. Gomes
12. Kinetic Analysis of Amyloid
... mehr
Formation
Georg Meisl, Thomas C.T. Michaels, Sara Linse, and Tuomas P. J. Knowles
13. Mapping Amyloid Regions in Gad m 1 with Peptide Arrays
Rosa Sánchez, Javier Martínez, Laura Montoya, Milagros Castellanos, and Maria Gasset
14. Non-Invasive Structural Analysis of Intermediate Species during Fibrillation: An Application of Small-Angle X-Ray Scattering
Annette Eva Langkilde, Fátima Herranz-Trillo, Pau Bernadó, and Bente Vestergaard
15. Analysis of Covalent Modifications of Amyloidogenic Proteins using Two-Dimensional Electrophoresis: Prion Protein and Its Sialylation
Elizaveta Katorcha and Ilia V. Baskakov
16. Amplification and Detection of Minuscule Amounts of Misfolded Prion Protein by Using The Real-Time Quaking-Induced-Conversion
Matthias Schmitz, Niccolò Candelise, Franc Llorens, and Inga Zerr
Part II: Cell Culture Models and Assays
17. Bacterial Amyloids
Margery L. Evans, Elizabeth Gichana, Yizhou Zhou, and Matthew R. Chapman
18. Addressing Intracellular Amyloidosis in Bacteria with RepA-WH1, a Prion-Like Protein
Laura Molina-García, Fátima Gasset-Rosa, María Moreno-del Álamo, Susana Moreno-Díaz de la Espina, and Rafael Giraldo
19. Study of Amyloids Using Yeast
Reed B. Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey, and Herman K. Edskes
20. Neurotoxic Ca2+ Signaling Induced by Amyloid-ss Oligomers in Aged Hippocampal Neurons In Vitro
Lucía Núñez, María Calvo-Rodríguez, Erica Caballero, Mónica García-Durillo, and Carlos Villalobos
21. Preparation and Culturing of Human Primary Vascular Cells
Finnbogi R. Thormodsson, Ingvar H. Olafsson, and Dadi Th. Vilhjalmsson
22. Live Imaging of Pathological Tau Protein and Tau Antibodies in a Human Neuron-Like Cellular Model
Dov B. Shamir, Yan Deng, and Einar M. Sigurdsson
23. Effects of Amyloid-ss Peptide on the Biology of Human Neural Stem Cells
Adela Bernabeu-Zornoza, Raquel Coronel, María Lachgar, Charlotte Palmer, and Isabel Liste
Part III: In Vivo Models and Assays
24. Development of Mouse Monoclonal Antibodies against Human Amyloid Fibril Proteins for Diagnostic and Research Purposes
Gunilla T. Westermark, Elisabet Ihse, and Per Westermark
25. Identification and Characterization of Amyloid-ss Accumulation in Synaptic Mitochondria
Shi Fang Yan, Firoz Akhter, Alexander A. Sosunov, and Shirley ShiDu Yan
26. Biochemical Properties of Pathology-Related Tau Species in Tauopathy Brains: An Extraction Protocol for Tau Oligomers and Aggregates
Naruhiko Sahara and Taeko Kimura
27. Tau Assembly into Filaments
Mar Pérez, Raquel Cuadros, and Miguel Medina
28. Quantitative Metabolomics in Alzheimer¿s Disease: Technical Considerations for Improved Reproducibility
Sergio Veiga, Judith Wahrheit, Andrés Rodríguez-Martín, and Denise Sonntag
29. Detecting Circulating MicroRNAs as Biomarkers in Alzheimer's Disease
Aidan Kenny, Eva M. Jimenez-Mateos, Miguel Calero, Miguel Medina, and Tobias Engel
30. Luminescent Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues
K. Peter R. Nilsson, Mikael Lindgren, and Per Hammarström
31. Characterization of Amyloid-ss Plaques and Autofluorescent Lipofuscin Aggregates in Alzheimer¿s Disease Brain: A Confocal Microscopy Approach
Alejandra Kun, Fernando González-Camacho, Silvia Hernández, Alexandra Moreno-García, Olga Calero, and Miguel Calero
32. In Vivo Imaging of Tauopathy in Mice
Senthilkumar Krishnaswamy, Qian Wu, Yan Lin, Wajitha J. Rajamohamedsait, Hameetha B. Rajamohamedsait, and Einar M. Sigurdsson
33. In Vivo Evaluation of Neuronal Transport in Murine Models of Neurodegeneration using Manganese-Enhanced MRI
Anne Bertrand, Maria Baron, Dung M. Hoang, Lindsay K. Hill, Sebastian L. Mendoza, Einar M. Sigurdsson, and Youssef Z. Wadghiri
Georg Meisl, Thomas C.T. Michaels, Sara Linse, and Tuomas P. J. Knowles
13. Mapping Amyloid Regions in Gad m 1 with Peptide Arrays
Rosa Sánchez, Javier Martínez, Laura Montoya, Milagros Castellanos, and Maria Gasset
14. Non-Invasive Structural Analysis of Intermediate Species during Fibrillation: An Application of Small-Angle X-Ray Scattering
Annette Eva Langkilde, Fátima Herranz-Trillo, Pau Bernadó, and Bente Vestergaard
15. Analysis of Covalent Modifications of Amyloidogenic Proteins using Two-Dimensional Electrophoresis: Prion Protein and Its Sialylation
Elizaveta Katorcha and Ilia V. Baskakov
16. Amplification and Detection of Minuscule Amounts of Misfolded Prion Protein by Using The Real-Time Quaking-Induced-Conversion
Matthias Schmitz, Niccolò Candelise, Franc Llorens, and Inga Zerr
Part II: Cell Culture Models and Assays
17. Bacterial Amyloids
Margery L. Evans, Elizabeth Gichana, Yizhou Zhou, and Matthew R. Chapman
18. Addressing Intracellular Amyloidosis in Bacteria with RepA-WH1, a Prion-Like Protein
Laura Molina-García, Fátima Gasset-Rosa, María Moreno-del Álamo, Susana Moreno-Díaz de la Espina, and Rafael Giraldo
19. Study of Amyloids Using Yeast
Reed B. Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey, and Herman K. Edskes
20. Neurotoxic Ca2+ Signaling Induced by Amyloid-ss Oligomers in Aged Hippocampal Neurons In Vitro
Lucía Núñez, María Calvo-Rodríguez, Erica Caballero, Mónica García-Durillo, and Carlos Villalobos
21. Preparation and Culturing of Human Primary Vascular Cells
Finnbogi R. Thormodsson, Ingvar H. Olafsson, and Dadi Th. Vilhjalmsson
22. Live Imaging of Pathological Tau Protein and Tau Antibodies in a Human Neuron-Like Cellular Model
Dov B. Shamir, Yan Deng, and Einar M. Sigurdsson
23. Effects of Amyloid-ss Peptide on the Biology of Human Neural Stem Cells
Adela Bernabeu-Zornoza, Raquel Coronel, María Lachgar, Charlotte Palmer, and Isabel Liste
Part III: In Vivo Models and Assays
24. Development of Mouse Monoclonal Antibodies against Human Amyloid Fibril Proteins for Diagnostic and Research Purposes
Gunilla T. Westermark, Elisabet Ihse, and Per Westermark
25. Identification and Characterization of Amyloid-ss Accumulation in Synaptic Mitochondria
Shi Fang Yan, Firoz Akhter, Alexander A. Sosunov, and Shirley ShiDu Yan
26. Biochemical Properties of Pathology-Related Tau Species in Tauopathy Brains: An Extraction Protocol for Tau Oligomers and Aggregates
Naruhiko Sahara and Taeko Kimura
27. Tau Assembly into Filaments
Mar Pérez, Raquel Cuadros, and Miguel Medina
28. Quantitative Metabolomics in Alzheimer¿s Disease: Technical Considerations for Improved Reproducibility
Sergio Veiga, Judith Wahrheit, Andrés Rodríguez-Martín, and Denise Sonntag
29. Detecting Circulating MicroRNAs as Biomarkers in Alzheimer's Disease
Aidan Kenny, Eva M. Jimenez-Mateos, Miguel Calero, Miguel Medina, and Tobias Engel
30. Luminescent Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues
K. Peter R. Nilsson, Mikael Lindgren, and Per Hammarström
31. Characterization of Amyloid-ss Plaques and Autofluorescent Lipofuscin Aggregates in Alzheimer¿s Disease Brain: A Confocal Microscopy Approach
Alejandra Kun, Fernando González-Camacho, Silvia Hernández, Alexandra Moreno-García, Olga Calero, and Miguel Calero
32. In Vivo Imaging of Tauopathy in Mice
Senthilkumar Krishnaswamy, Qian Wu, Yan Lin, Wajitha J. Rajamohamedsait, Hameetha B. Rajamohamedsait, and Einar M. Sigurdsson
33. In Vivo Evaluation of Neuronal Transport in Murine Models of Neurodegeneration using Manganese-Enhanced MRI
Anne Bertrand, Maria Baron, Dung M. Hoang, Lindsay K. Hill, Sebastian L. Mendoza, Einar M. Sigurdsson, and Youssef Z. Wadghiri
... weniger
Bibliographische Angaben
- 2018, 3. Aufl., XVII, 547 Seiten, 65 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Einar M. Sigurdsson, Miguel Calero, María Gasset
- Verlag: Springer, Berlin
- ISBN-10: 1493978152
- ISBN-13: 9781493978151
- Erscheinungsdatum: 11.06.2018
Sprache:
Englisch
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