Analysis and Function of Amino Acids and Peptides

MASS SPECTROMETRY OF AMINO ACIDS AND PROTEINSIntroductionBasic Protein Chemistry and How it Relates to MSSample Preparation and Data AcquisitionData Analysis of LC-MS/MS (or CE-MS/MS) of MixturesMS of Protein Structure, Folding, and InteractionsConclusions and PerspectivesX-RAY STRUCTURE DETERMINATION OF PROTEINS AND PEPTIDESIntroductionGrowing CrystalsSymmetry and Space GroupsX-Ray Scattering and DiffractionCollecting and Processing Diffraction DataSolving the Structure (Determining Phases)Analyzing and Refining the StructureNUCLEAR MAGNETIC RESONANCE OF AMINO ACIDS, PEPTIDES, AND PROTEINSIntroductionAmino AcidsPeptidesProteinsConclusionsSTRUCTURE AND ACTIVITY OF N-METHYLATED PEPTIDESIntroductionConformational Effects of N-MethylationEffects of N-Methylation on Bioactive PeptidesConcluding RemarksHIGH-PERFORMANCE LIQUID CHROMATOGRAPHY OF PEPTIDES AND PROTEINSIntroductionBasic Terms and Concepts in ChromatographyChemical Structure of Peptides and ProteinsHPLC Separation Modes in Peptide and Protein AnalysisMethod Development from Analytical to Preparative Scale Illustrated for HP-RPCMultidimensional HPLCConclusionsLOCAL SURFACE PLASMON RESONANCE AND ELECTROCHEMICAL BIOSENSING SYSTEMS FOR ANALYZING FUNCTIONAL PEPTIDESLocalized Surface Plasmon Resonance (LSPR)-Based Microfluidics Biosensor for the Detection of Insulin Peptide HormoneElectrochemical LSPR-Based Label-Free Detection of MelittinLabel-Free Electrochemical Monitoring of Beta-Amyloid (ABeta) Peptide AggragationSURFACE PLASMON RESONANCE SPECTROSCOPY IN THE BIOSCIENCESIntroductionSPR-Based Optical BiosensorsPrinciple of Operation of SPR BiosensorsDescription of a SPR InstrumentApplication of SPR in Immunosensor DesignApplication of SPR in Membrane InteractionsData AnalysisConclusionsATOMIC FORCE MICROSCOPY OF PROTEINSForewordAFMBioimaging HighlightsIssuesForce MeasurementsLiquid ImagingSample Preparation for BioimagingOutlookSOLVENT INTERACTIONS WITH PROTEINS AND OTHER MACROMOLECULESIntroductionSolvent

Andrew Hughes is a reader and associate professor of chemistry at La Trobe University, Melbourne, Australia. He obtained his degrees from the University of Western Australia, before taking up post-doctoral appointments at the University of Cambridge starting in 1989. After threeyears working with Professor Andrew Holmes, he joined Professor Steven Ley`s group. While at Cambridge he was appointed the Shell Research Fellow at Robinson College. His interests lie in the general fi eld of asymmetric synthesis and methodology, with a recent focus on amino acid chemistry and diffi cult peptides.